Effects of NMDA receptor antagonists MK-801 and CPPene on experimental spinal cord injury
Küçük Resim Yok
Tarih
1998
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
N-methyl-D-aspartate (NMDA) reseptör kökenli olayların omurilik yaralanmasının patofizyolojisinde rol aldığı bildirilmiştir. Bu çalışma, iki NMDA reseptör antagonistinin, CPPene (3-(2-carboxypiperazin-4-yl)-l-propenyl-1-phosphonicacid) ve MK-801 (dizocilpine maleate) beyaz sıçanlarda 50 gm-cm ağırlık düşürme omurilik travmasından sonraki etkilerini incelemektedir. Somatosensoriyel evoked potansiyeller (SEP) ve motor evoked potansiyeller (MEP) yaralanmadan hemen önce ve yaralanma sonrası 4 saat boyunca kayıtlanmıştır. Alt ekstremite motor skalaları travmadan sonraki l hafta boyunca eğimli tablada incelenmiştir. MK-801 (n=15) travmadan 30 dakika sonra l mg/kg dozda intraperitoneal olarak verilmiş, daha sonra da 75 dakika aralıklarla 0,5 mg/ kglık 3 rapel doz yapılmıştır. CPPene (n=10) travmadan 15 dakika sonra 4,5 mg/kg dozda verilmiş ve 3 saat sonra bu tekrarlanmıştır. Kontrol grup (n=12) salin enjeksiyonları almıştır. MK-801 grubu 15 hayvandan, CPPene grubu 10 hayvandan, kontrol grubu 12 hayvandan oluşmaktadır. Motor skala ve evoked potansiyel analizi grupları bilmeyen ikinci bir gözlemci tarafından yapılmıştır. Üç grup arasında motor skalalar ve MEPlerde bir fark bulunamamıştır. Ancak SEPler CPPene grubunda belirgin şekilde düzelmiştir. Bu sonuçlar CPPene in bu deneysel omurilik yaralanması modelinde dorsal kolon fonksiyonuna olumlu bir etkisi olduğunu göstermektedir.
N-methyl-D-aspartate(NMDA)receptor-mediated events have been implicated in the pathophysiology of spinal cord injury. This study examines the effects of two NMDA receptor antagonists, CPPene (3-(2-carboxypiperazin-4-yl)-1-propenyl-l-phosphonic acid) and MK-801 (dizocilpine maleate) after 50 gm-cm weight dropping spinal cord injury of white rats. Somatosensory evoked potentials (SEP) and motor evoked potentials (MEP) were recorded before and in different intervals for 4 hours after injury. Lower extremity motor scales were observed on inclined table for 1 week after injury. MK-801 (n=15) were given intraperitoneally first at 1 mg/kg doses 30 minutes after injury and 3 rapel doses of 0.5 mg/kg at 75 minutes intervals. CPPene (n=10) were given at 4.5 mg/kg doses 15 minutes after injury and it was repeated 3 hours after wards. Control group (n=12) had saline injections. MK-801 group consisted of 15 animals and CPPene group 10 animals. Motor scale and evoked potential analysis were done by the second observer not knowing the groups. There were no differences on motor scales and MEPs between three groups; however, SEPs significantly recovered in CPPene group. These results show that CPPene has a positive effect in dorsal column function on this experimental spinal cord injury.
N-methyl-D-aspartate(NMDA)receptor-mediated events have been implicated in the pathophysiology of spinal cord injury. This study examines the effects of two NMDA receptor antagonists, CPPene (3-(2-carboxypiperazin-4-yl)-1-propenyl-l-phosphonic acid) and MK-801 (dizocilpine maleate) after 50 gm-cm weight dropping spinal cord injury of white rats. Somatosensory evoked potentials (SEP) and motor evoked potentials (MEP) were recorded before and in different intervals for 4 hours after injury. Lower extremity motor scales were observed on inclined table for 1 week after injury. MK-801 (n=15) were given intraperitoneally first at 1 mg/kg doses 30 minutes after injury and 3 rapel doses of 0.5 mg/kg at 75 minutes intervals. CPPene (n=10) were given at 4.5 mg/kg doses 15 minutes after injury and it was repeated 3 hours after wards. Control group (n=12) had saline injections. MK-801 group consisted of 15 animals and CPPene group 10 animals. Motor scale and evoked potential analysis were done by the second observer not knowing the groups. There were no differences on motor scales and MEPs between three groups; however, SEPs significantly recovered in CPPene group. These results show that CPPene has a positive effect in dorsal column function on this experimental spinal cord injury.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Turkish Neurosurgery
WoS Q Değeri
Scopus Q Değeri
Cilt
8
Sayı
3-4
