Graphene oxide modified single-use electrodes and their application for voltammetric miRNA analysis
Küçük Resim Yok
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The modification of graphene oxide (GO) onto the surfaces of chemically activated pencil graphite electrodes (PGEs) was performed herein, and then these electrodes were applied for the first time on voltammetric monitoring of miRNAs. The specific recognition of miRNA-34a, which has been related to Alzheimer disease, was explored in the presence of DNA-RNA hybridization by using CA/GO/PGEs in combination with differential pulse voltammetry (DPV) technique. The characterization of CA/GO/PGE was investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The effect of GO concentration, DNA probe concentration and miRNA-34a concentration upon to biosensor response was optimized, and accordingly, the selectivity of miRNA-34a biosensor was tested under the optimum conditions. (C) 2017 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Graphene oxide, miRNA, Pencil graphite electrode, Differential pulse voltammetry, Electrochemical biosensor, Alzheimer disease
Kaynak
Materials Science & Engineering C-Materials For Biological Applications
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
75
