Investigation of lactic acid production by pressurized liquid hot water from cultivated Miscanthus x giganteus
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The production of lactic acid, a polylactic acid monomer from energy crop Miscanthus x giganteus lignocellulosic biomass cultivated in Izmir was investigated. Liquid hot water (LHW) pretreatment was carried out at a temperature range of 140-200 degrees C, pressure 100 to 200 bar and reaction time of 15-45 min at a fixed flow rate of 2 mL/min using D-optimal experimental plan. The optimum conditions were elicited as 140 degrees C, 100 bar and 45 minutes, yielding the highest reducing sugar content of 77.32 mg/g, whereas 1.25 mg/mL arabinose and 1.35 mg/mL xylose as monomeric sugars. Subsequently, the enzymatic hydrolysis was applied to the solid fraction. The optimum conditions for enzymatic hydrolysis were determined as 5% (w/v) solid/liquid ratio, 20 FPU/mL enzyme loading and 72 hours, revealing the highest amount of reducing sugar as 200 mg/mL. LHW hydrolysate was used as a production medium for lactic acid manufacturing in submerged fermentation by Rhizopus oryzae. The maximum lactic acid content was found to be 6.8 g/L at 24 hours, whereas the lactic acid yield was 0.28 g/L.h. The sequential design of LHW, followed by enzymatic hydrolysis and submerged lactic acid fermentation can be utilized in industry, contributing to the bioeconomy.
Açıklama
Anahtar Kelimeler
Miscanthus x giganteus, biorefinery, lactic acid, enzymatic hydrolysis, fermentation, biomass, Bioethanol Production, Wheat-Straw, Pretreatment, Fermentation, Hydrolysis, Inhibitors, Bacteria, Optimization, Conversion, Residues
Kaynak
Preparative Biochemistry & Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
