A Study of the Alkaloids in callusing plant tissues from a range of Turkish cultivars of Papaver somniferum.
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Tarih
1999
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info:eu-repo/semantics/openAccess
Özet
Petal rengi farklı 5 değişik Papaver somniferum L. tohumlarından (Emiral/84, Papaver somniferum var anatolicum) doku kültürü ile kallus üretilmesi ve üretilen bu kalluslarda alkaloit sentezinin kontrolu üzerinde çalışıldı. Çalışma sırasında örneklerden alınan ekstrelerde sekonder metabolit olarak alkaloitlerin varlığı ince tabaka kromatografisi, ve kütle spektrostopisi kullanılarak tarandı. Çalışma sırasında, 0.1 mg/l kinetin yanında iki değişik dozda (1.0 ve 5.0 mg/l) 2, 4-diklorofenoksiasetik asit (2, 4-D) ilavesi ile hazırlanmış Murashige & Skoog ve Gamborg B5 besi ortamları kullanıldı. 1.0 mg/l 2, 4-D hormon dozunda daha önceden çimlendirilerek her iki besi ortamına aktarılmış olan tohumlar sürgün verirken, 5.0 mg/l 2, 4-D hormon dozunda kallus oluşumu görüldü. Yapılan kimyasal analizler sonucunda 5.0 mg/l 2, 4-D hormon dozunda ve Murashige & Skoog besi ortamında yetiştirilen 1 nolu varyeteden elde edilen kallusda Dragendorff ile pozitif leke elde edildi.
Plant tissue cultures were obtained from the seeds of five varieties on the basis of petal colour) of Papaver somniferum and alkaloidal extracts were screend for the presence of secondary metabolites using thin layer chromatography and mass spectroscopy. Two plant tissue culture media were used in this study, Murashige & Skoog and Gamborg B5, supplemented with 0.1 mg/l of kinetin in addition to one of either 1.0 or 5.0 mg/l of 2, 4-dichlorophenoxyacetic acid (2, 4-D). Seedlings growing on media with 1.0 mg/l 2, 4-D produced planlets, while those supplemented with the higher dose, 5.0 mg/l, produced calluses. Variations were found in cultures produced from different seeds and in these grown on different media containing different levels of plant-growth hormones. Although all the original seed material contained unidentified non polar Dragendorff positive spots when examined using thin layer chromatography, none could be detected in either the calluses or plantlets produced from them. All five varieties of seed were grown on the four different media but only one, the callus derived from seed variety No. 1 and grown on a Murashige & Skoog medium supplemnetd with 0.1 mg/l kinetin and 5.0 mg/l 2, 4-D, contained one Dragendorff positive spot, which did not correspond to any of the standard alkaloids used, and was not identified by mass spectroscopy
Plant tissue cultures were obtained from the seeds of five varieties on the basis of petal colour) of Papaver somniferum and alkaloidal extracts were screend for the presence of secondary metabolites using thin layer chromatography and mass spectroscopy. Two plant tissue culture media were used in this study, Murashige & Skoog and Gamborg B5, supplemented with 0.1 mg/l of kinetin in addition to one of either 1.0 or 5.0 mg/l of 2, 4-dichlorophenoxyacetic acid (2, 4-D). Seedlings growing on media with 1.0 mg/l 2, 4-D produced planlets, while those supplemented with the higher dose, 5.0 mg/l, produced calluses. Variations were found in cultures produced from different seeds and in these grown on different media containing different levels of plant-growth hormones. Although all the original seed material contained unidentified non polar Dragendorff positive spots when examined using thin layer chromatography, none could be detected in either the calluses or plantlets produced from them. All five varieties of seed were grown on the four different media but only one, the callus derived from seed variety No. 1 and grown on a Murashige & Skoog medium supplemnetd with 0.1 mg/l kinetin and 5.0 mg/l 2, 4-D, contained one Dragendorff positive spot, which did not correspond to any of the standard alkaloids used, and was not identified by mass spectroscopy
Açıklama
Anahtar Kelimeler
Orman Mühendisliği
Kaynak
Turkish Journal of Agriculture and Forestry
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Cilt
23
Sayı
4
