Exposure of Porphyromonas gingivalis to cortisol increases bacterial growth
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pergamon-Elsevier Science Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: Psychological stress is considered as a risk factor for periodontal diseases. The stress-related hormone, cortisol is one of the main molecules released during human stress response and is found in plasma and gingival crevicular fluid. This hormone has been suggested to modify composition of subgingival biofilms. The aim of this study was to investigate the effect of exposure to cortisol on Porphyrornonas gingivalis (P. gingivalis) growth. Materials and methods: P. gingivalis ATCC strain 33277 was cultured under strict anaerobic conditions at 37 degrees C in Brain Heart Infusion medium supplemented with hemin (5 mu g ml(-1)) and menadione (1 mu g ml(-1)). Bacterial cultures were incubated with or without hydrocortisone (0.04-10 mu g ml(-1)) at 37 degrees C for 12, 24 and 48 h and bacterial growth was evaluated by spectrophotometric method (OD600 nm). Cortisol consumption has been followed by HPLC. Results: Cortisol significantly increased P. gingivalis growth in the first 24 h peaking at 12 h but this increase was not related to the concentration used. During the time period, no consumption of cortisol was observed. Conclusions: This study provides further support for the idea that stress-induced hormone; cortisol may influence the growth of P. gingivalis. This specific effect may be involved in the relationship between stress and periodontal diseases. (C) 2013 Elsevier Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
Cortisol, Periodontal disease, Porphyromonas gingivalis, Psychological stress
Kaynak
Archives of Oral Biology
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
59
Sayı
1
