Electrochemical detection of interaction between capsaicin and nucleic acids in comparison to agarose gel electrophoresis
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, the biomolecular interaction occurred between nucleic acids and Capsaicin (CPS), the active compound in chilli peppers, which has been reported to have anti-carcinogenic properties, was investigated for the first time herein using disposable electrochemical biosensor. It is aimed to perform the surface-confined interaction between CPS and different types of nucleic acids and under this aim, the experimental conditions were optimized; such as, the concentration of CPS and DNA, DNA immobilization time and interaction time etc. The detection limit of DNA was estimated based on guanine oxidation signal in the linear concentration range of DNA from 1 to 5 mu g/mL, and it was found to be 0.62 mu g/mL. The effect of time-dependent manner from 1 min to 30 min on the interaction of CPS with nucleic acids was explored upon to the changes at guanine signal coming from double stranded DNA and cDNA as well as PCR samples. The interaction of CPS with double stranded DNA was also determined by agarose gel electrophoresis. (C) 2017 Elsevier Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Capsaicin, DNA interaction, Electrochemical DNA biosensor, Differential pulse voltammetry, Pencil graphite electrode, Agarose gel electrophoresis
Kaynak
Analytical Biochemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q3
Cilt
535
