Radiolabeled methotrexate loaded chitosan nanoparticles as imaging probe for breast cancer: Biodistribution in tumor-bearing mice
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
There is great interest in developing nanoparticle drug delivery systems for the delivery of therapeutics or im-aging agents for cancer. In this context, a new radiopharmaceutical was prepared by labeling the methotrexate (MTX) loaded chitosan nanoparticles (CSNPs) with [99mTc]Tc radionuclide, and the breast cancer imaging po-tential of the radiopharmaceutical was determined by in-vivo biodistribution studies. Within the scope, different amounts of MTX-loaded CSNPs (MTX-CSNPs) (F1-F3) were prepared via an ionotropic gelation process. The physicochemical properties of MTX-CSNPs were evaluated. The mean diameter of MTX-CSNPs was found be-tween 148.8 +/- 0.636 nm and 157.0 +/- 3.960 nm, the PdI value of between 0.415 +/- 0.042 and 0.451 +/- 0.036 and the zeta potential of between 15.7 +/- 1.17 mV and 18.7 +/- 0.27 mV. In addition, the preparation efficiency of MTX-CSNPs was found to be between 64.036 +/- 2.054% -70.234 +/- 2.140% and the encapsulation efficiency of CSNPs was found to be between 32.301 +/- 1.205-50.304 +/- 2.478%. MTX-CSNPs were radiolabeled with [99mTc] Tc, and the radiolabeled nanoparticles ([99mTc]Tc-MTX-CSNPs) were evaluated by both in-vitro cell binding studies and in-vivo biodistribution studies. [99mTc]Tc-MTX-CSNPs showed high labeling efficiency (>= 99%) and in-vitro stability up to 6 h at room temperature. According to the cell incorporation study, [99mTc]Tc-MTX-CSNPs (F3) showed significantly higher uptake into 4T1 (breast cancer) cells than R/H-[99mTc]Tc. Biodistribution studies of [99mTc]Tc-MTX-CSNPs (F3) were performed in breast tumor-bearing female Balb/c mice. Compared to [99mTc]Tc-MIBI, [99mTc]Tc-MTX-CSNPs (F3) exhibited significantly higher uptake by breast tumors. This study demonstrates the affinity of [99mTc]Tc-MTX-CSNPs to breast cancer cells and their diagnostic utility.
Açıklama
Anahtar Kelimeler
Biodistribution, Breast cancer, Chitosan nanoparticles, Methotrexate, Radiolabeling, Radiopharmaceuticals, Technetium-99 m, Computed-Tomography, Nanocarriers, Statistics, Chemistry, Delivery
Kaynak
Journal of Drug Delivery Science and Technology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
80
