Microorganism compositions for the degradation of lipids in wastewater
Yükleniyor...
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege üniversitesi, Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This thesis aimed to establish consortia of microorganisms that can degrade crude oil, diesel and vegetable oil efficiently. The degradation rates were determined using gravimetric analysis method. GC and HPLC were respectively used to determine the residual hydrocarbons and triacylglycerides. After sampling, the phylogenetic analyses classified the selected strains as Acinetobacter (2SA, 2SC, C1-8 and C1-9), Bacillus (7SA), Chryseobacterium (2SB) and Pseudomonas (NPK). 2SB, 7SA and NPK isolates displayed ability to produce biosurfactants. A consortium consisting of 2SA, C1-8, C1-9 and NPK was established for the degradation of oily sludge. Acinetobacter strains showed high degradation capability of alkanes, while PAHs were degraded by NPK and 2SA. The rate of oily sludge degradation by the consortium was optimum (93%) at 30°C, pH 7. The gDNA of strain 2SA revealed the presence three different alkane hydroxylases (AH) genes (alkB, almA and cyp153), while that of strains C1-8 and C1-9 harboured alkB and almA. The influence of kurstakin molecules on the ability of 2SA to degrade diesel oil was also evaluated. The results showed that the diesel oil degradation was less in the absence of kurstakin (70%) than its presence (90%). A consortium of 2SA, 2SB and 2SC was able to degrade 96% of olive oil after 14 days. The isolates recorded high lipase production using the tributyrin agar test.
This thesis aimed to establish consortia of microorganisms that can degrade crude oil, diesel and vegetable oil efficiently. The degradation rates were determined using gravimetric analysis method. GC and HPLC were respectively used to determine the residual hydrocarbons and triacylglycerides. After sampling, the phylogenetic analyses classified the selected strains as Acinetobacter (2SA, 2SC, C1-8 and C1-9), Bacillus (7SA), Chryseobacterium (2SB) and Pseudomonas (NPK). 2SB, 7SA and NPK isolates displayed ability to produce biosurfactants. A consortium consisting of 2SA, C1-8, C1-9 and NPK was established for the degradation of oily sludge. Acinetobacter strains showed high degradation capability of alkanes, while PAHs were degraded by NPK and 2SA. The rate of oily sludge degradation by the consortium was optimum (93%) at 30°C, pH 7. The gDNA of strain 2SA revealed the presence three different alkane hydroxylases (AH) genes (alkB, almA and cyp153), while that of strains C1-8 and C1-9 harboured alkB and almA. The influence of kurstakin molecules on the ability of 2SA to degrade diesel oil was also evaluated. The results showed that the diesel oil degradation was less in the absence of kurstakin (70%) than its presence (90%). A consortium of 2SA, 2SB and 2SC was able to degrade 96% of olive oil after 14 days. The isolates recorded high lipase production using the tributyrin agar test.
This thesis aimed to establish consortia of microorganisms that can degrade crude oil, diesel and vegetable oil efficiently. The degradation rates were determined using gravimetric analysis method. GC and HPLC were respectively used to determine the residual hydrocarbons and triacylglycerides. After sampling, the phylogenetic analyses classified the selected strains as Acinetobacter (2SA, 2SC, C1-8 and C1-9), Bacillus (7SA), Chryseobacterium (2SB) and Pseudomonas (NPK). 2SB, 7SA and NPK isolates displayed ability to produce biosurfactants. A consortium consisting of 2SA, C1-8, C1-9 and NPK was established for the degradation of oily sludge. Acinetobacter strains showed high degradation capability of alkanes, while PAHs were degraded by NPK and 2SA. The rate of oily sludge degradation by the consortium was optimum (93%) at 30°C, pH 7. The gDNA of strain 2SA revealed the presence three different alkane hydroxylases (AH) genes (alkB, almA and cyp153), while that of strains C1-8 and C1-9 harboured alkB and almA. The influence of kurstakin molecules on the ability of 2SA to degrade diesel oil was also evaluated. The results showed that the diesel oil degradation was less in the absence of kurstakin (70%) than its presence (90%). A consortium of 2SA, 2SB and 2SC was able to degrade 96% of olive oil after 14 days. The isolates recorded high lipase production using the tributyrin agar test.
Açıklama
Anahtar Kelimeler
Biodegradation, Crude Oil, Diesel Oil, Olive Oil, Biosurfactant, Biyodegradasyon, Ham Yağı, Dizel Yağı, Zeytinyağı, Biyosürfektan
