Laccase biosensors based on mercury thin film electrode
Küçük Resim Yok
Tarih
2005
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A biosensor was developed by immobilizing laccase onto mercury thin film electrode (MTFE) by means of gelatin that is then crosslinked with glutaraldehyde. Mercury thin film (MTF) was deposited onto glassy carbon electrode (GCE) and the obtained biosensor was utilized for the determination of phenolic compounds. The measurement was based on the amperometric detection of oxygen consumption in relation to analyte oxidation. The optimum experimental conditions for the biosensor were investigated and the system was calibrated for both catechol and phenol. A linear relationship between sensor responses and analyte concentrations was obtained in concentration range between 0.5 × 10-6-5.0 × 10-6 M for catechol and 2.5 × 10-6-2.0 × 10-6 M for phenol, respectively. Mercury thin film was also formed onto the surface of screen printed graphite electrodes and applied for the catechol detection. The linearity was observed in concentration range between 2.5 × 10-6-3.0 × 10 -5 M. Copyright © Taylor & Francis, Inc.
Açıklama
Anahtar Kelimeler
Biosensor, Laccase, Mercury thin film electrods
Kaynak
Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
33
Sayı
4
