Involvement of GLR-mediated nitric oxide effects on ROS metabolism in Arabidopsis plants under salt stress

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dc.contributor.authorGokce, Azime
dc.contributor.authorCetinel, Askim Hediye Sekmen
dc.contributor.authorTurkan, Ismail
dc.date.accessioned2024-08-31T07:47:05Z
dc.date.available2024-08-31T07:47:05Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractPlant glutamate receptor-like channels (GLRs) play important roles in plant development, immune response, defense signaling and Nitric oxide (NO) production. However, their involvement in abiotic stress responses, particularly in regulating Reactive Oxygen Species (ROS), is not well understood. This study aimed to investigate GLR-mediated NO production on ROS regulation in salt-stressed cells. To achieve this, Arabidopsis thaliana Columbia (Col-0) were treated with NaCl, glutamate antagonists [(DNQX (6,7-dinitroquinoxaline-2,3-dione and AP-5(D-2-amino-5-phosphono pentanoic acid)], and NO scavenger [cPTIO (2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt)]. Salt-stressed plants in combination with DNQX and AP-5 have exhibited higher increase in lipid peroxidation (TBARS), hydrogen peroxide (H2O2) and superoxide radical (O-2) contents as compared to solely NaCl-treated plants. Furthermore, NO and total glutathione contents, and S-nitrosoglutathione reductase (GSNOR) activity decreased with these treatments. AP-5 and DNQX increased the activities of NADPH oxidase (NOX), catalase (CAT), peroxidase (POX), cell wall peroxidase (CWPOX) in salt-stressed Arabidopsis leaves. However, their activities (except NOX) were significantly inhibited by cPTIO. Conversely, the combination of NaCl and GLR antagonists, NO scavenger decreased the activities of ascorbate peroxidase (APX), superoxide dismutase (SOD), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) resulting in elevated GSSG levels, a low GSH/GSSG ratio, impaired ROS scavenging, excessive ROS accumulation and cell membrane damage. The findings of this study provide evidence that GLR-mediated NO plays a crucial role in improvement of the tolerance of Arabidopsis plants to salt-induced oxidative stress. It helps to maintain cellular redox homeostasis by reducing ROS accumulation and increasing the activity of SOD, GSNOR, and the ASC-GSH cycle enzymes.en_US
dc.description.sponsorshipEge University [2018-FEN-035]; Ege University Research Foundationen_US
dc.description.sponsorshipThis work was supported by the Ege University Research Foundation (2018-FEN-035).en_US
dc.identifier.doi10.1007/s10265-024-01528-1
dc.identifier.endpage503en_US
dc.identifier.issn0918-9440
dc.identifier.issn1618-0860
dc.identifier.issue3en_US
dc.identifier.pmid38448641en_US
dc.identifier.scopus2-s2.0-85186888107en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage485en_US
dc.identifier.urihttps://doi.org/10.1007/s10265-024-01528-1
dc.identifier.urihttps://hdl.handle.net/11454/104306
dc.identifier.volume137en_US
dc.identifier.wosWOS:001175357300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Japan Kken_US
dc.relation.ispartofJournal of Plant Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectAntioxidant Enzymesen_US
dc.subjectGlutamate Receptorsen_US
dc.subjectNitric Oxideen_US
dc.subjectReactive Oxygen Speciesen_US
dc.subjectSalt Stressen_US
dc.titleInvolvement of GLR-mediated nitric oxide effects on ROS metabolism in Arabidopsis plants under salt stressen_US
dc.typeArticleen_US

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