Oxidized carbon powder for enzyme immobilization on screen printed biosensors
Küçük Resim Yok
Tarih
2002
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
World Scientific Publ Co Pte Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Carbon powders were subjected to an oxidation treatment with hydrogen peroxide in order to create surface oxygen groups (mainly carboxylic and phenolic groups) on printed electrodes for further covalent immobilisation of biomolecules. The oxidizing treatment modifies the surface chemistry of the carbon by creating surface-oxygen complexes that make it more acidic. Furthermore, the pH value of the carbon slurry was found to be changed from 6.5 to 4.4 after oxidizing step which introduced acidic groups on the surface. The porous structure of the carbonaceous support was not affected by oxidation as resulted by scanning electron microscopy before and after the treatment. Printed electrode's were fabricated by depositing several ink layers on a PVC substrate. Different oxidized carbon powders (including rhodium, (10%), including dextran (25%) and lysine coupled graphite) were printed to obtain the working electrodes. Glucose oxidase was used as a model enzyme to test several immobilisation methods on the treated screen printed surfaces. Binding of the enzyme were performed on (1) EDC activated, (2) CDI activated surfaces and also, (3) Lysine coupled surfaces by using EDC activation, respectively. Moreover, storage stabilities at 4oC were investigated.
Açıklama
7th Italian Conference on Sensors and Microsystems -- FEB 04-MAY 06, 2002 -- Bologna, ITALY
Anahtar Kelimeler
Kaynak
Sensors and Microsystems
WoS Q Değeri
N/A
