The enhancement of polygalacturonase and polymethylgalacturonase production on solid-state conditions by Aspergillus foetidus
Küçük Resim Yok
Tarih
2008
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, a previously isolated strain of Aspergillus foetidus Ege-K-635 producing highly active polygalacturonase and polymethylgalacturonase was used for improvement of enzyme production using solid-state conditions. To enhance the enzyme yield, the mixture of two substrates such as sugar beet pulp and wheat bran, having different ratios of carbon-nitrogen and moisture levels was used. Further the effect of the amount of inoculum was investigated. Physical parameters for the highest polygalacturonase and polymethylgalacturonase production under solid state conditions were determined on wheat bran: the sugar beet pulp (1:2) mixture substrate composition having a 31:1 carbon-nitrogen ratio with 8 x 10(6) spores/gram of dry substrate as the inoculum amount. Adequate moisture levels were 75% and 70% for polygalacturonase and polymethylgalacturonase, respectively. Under these conditions, the highest activities obtained were 385 +/- 12.3 Ug(-1) on the third day for polygalacturonase and 18.3 +/- 3.2 Ug(-1) on the second day for polymethylgalacturonase.
Açıklama
Anahtar Kelimeler
enhancement of pectinase, polygalacturonase, polymethylgalacturonase, solid state, sugar beet pulp, Aspergillus foetidus Ege-K-635
Kaynak
Food Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
22
Sayı
3
