DNA sensing on glassy carbon electrodes by using hemin as the electrochemical hybridization label
Küçük Resim Yok
Tarih
2002
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer-Verlag Berlin
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The electrochemical behavior of hemin, an iron complex of porphyrin, on binding to DNA at a glassy carbon electrode (GCE) and in solution, is described. Hemin, which interacts with covalently immobilized calf thymus DNA, was detected by use of a bare GCE, a double-stranded DNA-modified GCE (dsDNA-modified GCE), and a single-stranded DNA-modified GCE (ssDNA-modified GCE), in combination with differential pulse voltammetry (DPV). The structural conformation of DNA was determined from changes in the voltammetric signals acquired on reduction of hemin. As a result of its large steric structure and anionic substitution on its porphyrin plane, hemin intercalates between the base pairs of dsDNA. A scan-rate study for hemin and the dsDNA-hemin complex were also performed to determine the electrochemical behavior of the complex. The partition coefficient was obtained from the peak currents measured when different concentrations of hemin were in the presence of dsDNA. By observing the oxidation signals of guanine, damage to DNA after reaction with hemin at the GCE surface was also detected. The electrochemical detection of hybridization between the covalently immobilized probe and its target sequence was detected by use of hemin. These results demonstrate the use of DNA biosensors in conjunction with hemin for electrochemical detection of hybridization and damage to DNA.
Açıklama
1st Workshop on Evaluation/Validation of Novel Biosensors in Real Environment and Food Sample -- MAY 24-27, 2001 -- ATHENS, GREECE
Anahtar Kelimeler
DNA biosensor, hemin, intercalator, damage, hybridization
Kaynak
Analytical and Bioanalytical Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
373
Sayı
8
