Isolation and characterization of amylase producing yeasts and improvement of amylase production [Amilaz Üreticisi Mayalari{dotless}n İzolasyonu ve Karakterizasyonu ve Amilaz Üretiminin Artti{dotless}ri{dotless}lmasi{dotless}]
Küçük Resim Yok
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objectives: Because most of the yeasts from nature are not harmful as compared to bacteria, interest in yeasts with potential use in biotechnological processes has increased in recent years. Therefore, in this study, we aimed to isolate and characterize amylase producing yeasts and investigate the effects of different parameters on the production of amylase. Methods: Five different sources were used for the isolation of the yeasts. For screening of the amylase production, the isolates were incubated in Amylase Activity Medium at 30°C for 3 days. The amylase activity was determined by dinitrosalicylic acid method. Molecular characterization of the amylase producing yeasts was performed with restriction fragment length polymorphism (RFLP) of the 5.8S and 18S rRNA regions, and sequence analysis of D1/D2 domain of the 26S rRNA region. Results: In total, twenty five yeast isolates were obtained from five different sources. Following the incubation in medium containing starch for the screening of amylase production, it was found that 12 yeast isolates produce amylase, and among the isolates, three of them showed the highest amylase activity. The isolates (19-3, 19-6 and 19-7) having the highest activity were identified as S. fibuligera. The strains showed the highest amylase production at 30°C and pH 5.5. Among additives, sodium chloride, polyethylene glycol, sodium dodecyl sulfate, Triton X-100 and Tween 80 stimulated the production of amylase. Other additives showed negative influence on the production of enzyme. Conclusion: The study revealed that favorable fermentation conditions and the selection of suitable growth parameters played key roles in the production of amylase by S. fibuligera. © TurkJBiochem.com.
Açıklama
Anahtar Kelimeler
Improvement of amylase production, Molecular characterization, Saccharomycopsis fibuligera, Yeast
Kaynak
Turkish Journal of Biochemistry
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
38
Sayı
1
