Investigation of enteropathogenic Escherichia coli and Shiga toxin-producing Escherichia coli associated with hemolytic uremic syndrome in İzmir Province, Turkey

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dc.contributor.authorElif Bozçal
dc.contributor.authorGürkan Yiğittürk
dc.contributor.authorAtaç Uzel
dc.contributor.authorSabire Şöhret Aydemir
dc.date.accessioned2019-10-26T19:29:19Z
dc.date.available2019-10-26T19:29:19Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground/aim: The purpose of this study was to investigate Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains originating from diarrheagenic patients. Materials and methods: A total of 102 patients with diarrhea between October 2012 and January 2013 were enrolled in this study. Multiplex and standard polymerase chain reactions were performed to detect and distinguish STEC and EPEC strains. O serotyping of EPEC was carried out by monovalent antisera. The O and H serotyping of STEC strains was performed at the Refik Saydam Institute, Ankara. Results: A total of 5 (3.42%) strains were identified as STEC, and 3 strains (2.05%) were atypical EPEC. One of the STEC serotypes was O157:H7 carrying VT1, Stx1A, and escv genes. The other STEC strain was identified as O174:H21, which is associated with hemolytic uremic syndrome and consists of VT2 and Stx2A genes. One of the EPEC and three of the STEC serotypes were nontypeable. The serotypes of the atypical EPEC strains were identified as O114 and O26. Conclusion: To the best of our knowledge, this is the first report of O174:H21 from the İzmir region that was shown to be a Shiga toxinproducing non-O157 serotype of STEC.en_US
dc.description.abstractBackground/aim: The purpose of this study was to investigate Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains originating from diarrheagenic patients. Materials and methods: A total of 102 patients with diarrhea between October 2012 and January 2013 were enrolled in this study. Multiplex and standard polymerase chain reactions were performed to detect and distinguish STEC and EPEC strains. O serotyping of EPEC was carried out by monovalent antisera. The O and H serotyping of STEC strains was performed at the Refik Saydam Institute, Ankara. Results: A total of 5 (3.42%) strains were identified as STEC, and 3 strains (2.05%) were atypical EPEC. One of the STEC serotypes was O157:H7 carrying VT1, Stx1A, and escv genes. The other STEC strain was identified as O174:H21, which is associated with hemolytic uremic syndrome and consists of VT2 and Stx2A genes. One of the EPEC and three of the STEC serotypes were nontypeable. The serotypes of the atypical EPEC strains were identified as O114 and O26. Conclusion: To the best of our knowledge, this is the first report of O174:H21 from the İzmir region that was shown to be a Shiga toxinproducing non-O157 serotype of STEC.en_US
dc.identifier.endpage741en_US
dc.identifier.issn1300-0144
dc.identifier.issue3en_US
dc.identifier.startpage733en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpRNE5UUTRPQT09
dc.identifier.urihttps://hdl.handle.net/11454/10536
dc.identifier.volume46en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US]
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCerrahien_US
dc.titleInvestigation of enteropathogenic Escherichia coli and Shiga toxin-producing Escherichia coli associated with hemolytic uremic syndrome in İzmir Province, Turkeyen_US
dc.typeArticleen_US

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