Acyl chain length and charge effect on Tamoxifen-lipid model membrane interactions

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dc.contributor.authorBilge, Duygu
dc.contributor.authorKazanci, Nadide
dc.contributor.authorSevercan, Feride
dc.date.accessioned2019-10-27T21:52:10Z
dc.date.available2019-10-27T21:52:10Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractTamoxifen (TAM), which is an antiestrogenic agent, is widely used during chemotherapy of breast, pancreas, brain and liver cancers. In this study, TAM and model membrane interactions in the form of multilamellar vesicles (MLVs) were studied for lipids containing different acyl chain length and different charge status as a function of different TAM (1, 6, 9 and 15 mol%) concentrations. Zwitterionic lipids namely dipalmitoyl phosphatidylcholine (DPPC), and dimyristoylphosphatidylcholine (DMPC) lipids were used to see the acyl chain length effect and anionic dipalmitoyl phosphtidylglycerol (DPPG) lipid was used to see the charge effect. For this purpose Fourier transform-infrared (FTIR) spectroscopic and differential scanning calorimetric (DSC) techniques have been conducted. For zwitterionic lipid, concentration dependent different action of TAM was observed both in the gel and liquid crystalline phases by significantly increasing the lipid order and decreasing the dynamics for 1 mol% TAM, while decreasing the lipid order and increasing the dynamics of the lipids for higher concentrations (6, 9 and 15 mol%). However, different than neutral lipids, the dynamics and disorder of DPPG liposome increased for all TAM concentrations. The interactions between TAM and head group of multilamellar liposomes was monitored by analyzing the C=O stretching and PO2- antisymmetric double bond stretching bands. Increasing Tamoxifen concentrations led to a dehydration around these functional groups in the polar part of the lipids. DSC studies showed that for all types of lipids, TAM eliminates the pre-transition, shifts the main phase transition to lower temperatures and broadened the phase transition curve. The results indicate that not the acyl chain length but the charge status of the polar head group induces different effects on lipid membranes order and dynamics. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipEge University Research FundEge Universityen_US
dc.description.sponsorshipThis work was supported by Ege University Research Fund 2007 Fen 039.en_US
dc.identifier.doi10.1016/j.molstruc.2013.02.031
dc.identifier.endpage82en_US
dc.identifier.issn0022-2860
dc.identifier.issn1872-8014
dc.identifier.issn0022-2860en_US
dc.identifier.issn1872-8014en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage75en_US
dc.identifier.urihttps://doi.org/10.1016/j.molstruc.2013.02.031
dc.identifier.urihttps://hdl.handle.net/11454/47520
dc.identifier.volume1040en_US
dc.identifier.wosWOS:000318961000011en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Molecular Structureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTamoxifenen_US
dc.subjectFTIRen_US
dc.subjectDSCen_US
dc.subjectChain lengthen_US
dc.subjectCharge effecten_US
dc.titleAcyl chain length and charge effect on Tamoxifen-lipid model membrane interactionsen_US
dc.typeArticleen_US

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