A novel functional conducting polymer: synthesis and application to biomolecule immobilization

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dc.contributor.authorKanik, Fulya Ekiz
dc.contributor.authorRende, Eda
dc.contributor.authorTimur, Suna
dc.contributor.authorToppare, Levent
dc.date.accessioned2019-10-27T21:40:49Z
dc.date.available2019-10-27T21:40:49Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractA recently synthesized conducting polymer poly(TBT6-NH2); poly(6-(4,7-di(thiophen-2-yl)-2H-benzo [d][1,2,3]triazol-2-yl)hexan-1-amine) was utilized as a matrix for biomolecule immobilization. After successful electrochemical deposition the polymer poly(TBT6-NH2) on the graphite electrodes, immobilization of choline oxidase (ChO) was carried out. Due to the free amino functional groups of the polymeric structure, ChO molecules were successfully immobilized onto the polymer surface via covalent binding. For this, glutaraldehyde (GA) was used as crosslinker and bifunctional agent. Hence, a robust binding between the support and the protein molecules was achieved. Scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) were used to monitor the surface morphologies of both the polymer and the bioactive layer and to confirm the binding of the protein. Amperometric measurements were recorded by monitoring oxygen consumption in the presence of choline as the substrate at -0.7 V. The optimized biosensor showed a very good linearity between 0.1 and 10 mM with a 7 s response time and a detection limit (LOD) of 16.8 mu M to choline. Also, kinetic parameters, operational and storage stabilities were determined. Finally, designed system was applied for pesticide detection.en_US
dc.description.sponsorshipTUBATurkish Academy of Sciences; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110T580]en_US
dc.description.sponsorshipThis work is partially supported by TUBA and TUBITAK 110T580 grants. METU Central Laboratory is acknowledged for the SEM and XPS analyses.en_US
dc.identifier.doi10.1039/c2jm34100j
dc.identifier.endpage22525en_US
dc.identifier.issn0959-9428
dc.identifier.issn1364-5501
dc.identifier.issn0959-9428en_US
dc.identifier.issn1364-5501en_US
dc.identifier.issue42en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage22517en_US
dc.identifier.urihttps://doi.org/10.1039/c2jm34100j
dc.identifier.urihttps://hdl.handle.net/11454/46386
dc.identifier.volume22en_US
dc.identifier.wosWOS:000310721300027en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofJournal of Materials Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleA novel functional conducting polymer: synthesis and application to biomolecule immobilizationen_US
dc.typeArticleen_US

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