Brine shrimp lethality bioassay on some ranunculus species

The cytotoxic activity of Ranunculus pedatus Waldst.&Kit. subsp. pedatus growing wildly in Spil (Manisa), R. constantinapolitanus DC. growing wildly in Yamanlar (İzmir), R. isthmicus Boiss. subsp. tenuifolius growing wildly in Bozdag (İzmir) respectively were determined. LC 50 value of n-hexane, ethyl acetate, ethanol, methanol and water extracts of the plant materials were determined using brine shrimp (Artemia salina) lethality bioassay. The n-hexane, ethyl acetate, methanol and water extracts of R.pedatus Waldst.&Kit. subsp. pedatus, the n-hexane, ethyl acetate and water extracts of R. constantinapolitanus DC. and the n-hexane, ethanol and water extracts of R. isthmicus Boiss. subsp. tenuifolius showed cytotoxic activity against the Brine shrimp.