Fluctuation of "sperm DNA integrity" in accordance with semen parameters, and it's relationship with infertility
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer/Plenum Publishers
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose Nowadays sperm analysis is used routinely to assess infertile men, but it's predictive value is still restricted. For this reason, new markers are needed for diagnosis and guide treatment. A DNA damage evaluation method, which is easily applicable for routine analysis is described in this paper to provide an important support in clinical evaluation. Methods In this study, DNA damage evaluation was planned in infertile males with the COMET assay, a method which is simple, cheap, sensitive and reliable. For this purpose, the sperm DNA damage of normospermic, oligospermic, asthenospermic and teratospermic cases were investigated and the relationship between sperm parameters (sperm count, motility, morphology) and DNA damage was assessed. Results and conclusion According to our results we suggest that the factors to blame for sperm DNA damage and semen quality are the same. Also it was found that the infertile males possess substantially more sperm DNA damage than fertile men do. Improving and using the DNA damage evaluation tests seem to improve the results in ART clinics.
Açıklama
Anahtar Kelimeler
Assisted reproductive technologies (ART), COMET assay, Male infertility, Sperm, DNA damage
Kaynak
Journal of Assisted Reproduction and Genetics
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
31
Sayı
12
