Non-invasive electrochemical immunosensor for reverse iontophoretic determination of cardiac troponins (cTnT & cTnI) in a simulated artificial skin model. Significance of raw DPV and CV data for chemometric discrimination
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Electrochemical immunosensors coupled with reverse iontophoresis (RI) for noninvasive determination of cardiac troponins were developed and validated according to ICH Q2 (R1) guideline. Linearity was in 0.01-10 and 100-500 ng/mL ranges. LODs (ng/mL) were in 6-25 x 10-4, while LOQs (mu g/mL) were in 18-7.5 x 10-4 range. Chemometric evaluation was performed on raw data simply by principle component analysis and cluster analysis to discriminate stages of immunosensors. This is the first demonstration of RI determination of cardiac troponins so far. Findings of the current manuscript have great potential to develop point of care diagnostic systems for major cardiac events, where high sensitivity and specificity are required.
Açıklama
Anahtar Kelimeler
Cardiac troponin T, Cardiac troponin I, Chemoinformatics, Immunosensor, Reverse iontophoresis, Determining Electroosmotic Flow, Label-Free, Glucose, Lactate, Ph
Kaynak
Talanta
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
256
