Developmental expression of ecdysone-related genes associated with metamorphic changes during midgut remodeling of silkworm bombyx mori (Lepidoptera:Bombycidae)
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Library of the University of Arizona
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Steroid hormone 20-hydroxyecdysone is known as the systemic regulators of insect cells; however, how to impact the fate and function of mature and stem cells is unclear. For the first time, we report ecdysone regulatory cascades in both mature midgut cell and stem cell fractions related to developmental events by using histological, immunohistochemical, biochemical and gene expression analysis methods. Ecdysone receptor-B1 (EcRB1) and ultraspiracle 1 (USP-1) mRNAs were detected mainly in mature cells during programmed cell death (PCD). Lowered E75A and probably BR-C Z4 in mature cells appear to provide a signal to the initiation of PCD. E74B, E75B and BR-C Z2 seem to be early response genes which are involved in preparatory phase of cell death. It is likely that bFTZ-F1, E74A and BR-C Z1 are probably associated with execution of death. EcR-A and USP2 mRNAs were found in stem cells during remodeling processes but EcR-B1, USP1 and E74B genes imply an important role during initial phase of metamorphic events in stem cells. BHR3 mRNAs were determined abundantly in stem cells suggesting its primary role in differentiation. All of these results showed the determination the cell fate in Bombyx mori (Linnaeus) midgut depends on type of ecdysone receptor isoforms and ecdysonerelated transcription factors. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America.
Açıklama
Anahtar Kelimeler
Bombyx mori, Ecdysone-related gene, Midgut, Programmed cell death, Stem cell
Kaynak
Journal of Insect Science
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
16
Sayı
1
