Analytical Grade Purification of Phycocyanin from Cyanobacteria
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Humana Press Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Phycocyanin is a blue-colored pigment-protein complex that exhibits numerous biofunctions such as anti-inflammation, antioxidation, antitumor, neuroprotective effect, and immunological enhancement. Purified phycocyanin has pharmaceutical and nutraceutical applications. In addition, as a nontoxic and non-carcinogenic natural coloring agent, phycocyanin has many applications in the food and cosmetic industries. This chapter describes a protocol for extraction and analytical grade purification of phycocyanin from cyanobacteria. The purification steps include (1) extraction of phycocyanin from biomass, (2) ammonium sulfate precipitation of phycocyanin and dialysis, and (3) purification of phycocyanin by gel filtration and ion-exchange chromatography. © 2018, Springer Science+Business Media New York.
Açıklama
Anahtar Kelimeler
Cyanobacteria, Extraction, Gel filtration chromatography, Ion-exchange chromatography, Phycocyanin, Purification
Kaynak
Methods in Molecular Biology
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
1980
