Deneysel olarak oluşturulmuş akut pankreatitte kolonik değişiklikler
Küçük Resim Yok
Tarih
1998
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu deneysel çalışma Ege Üniversitesi Tıp Fakültesi Genel Cerrahi Kliniği'nin Deneysel Cerrahi Ünitesinde gerçekleştirildi. Yirmibir albino sıçan, kontrol A grubu (n=7), pankreatit oluşturulan B1 (n=7) ve B2 (n=7) deney grupları olmak üzere üç gruba ayrıldı. 20 $mu$g/kg cerulein, hafiften ciddiye kadar pankreatit oluşturmak amacıyla farklı protokollerde subkutan olarak uygulandı. A grubuna 2 cc % 0.9 NaCl subkutan olarak enjekte edildi ve hayvanlar 24 saat sonra eter anestezisi ile sakrifiye edildiler. Cerulein, 20 $mu$g/kg, SC olarak B1 grubuna tek defada, B2 grubuna yedi gün arayla dört kez uygulandı. Deneklerden, 15 gün sonra eter anestezisi ile sakrifiye edilmeden hemen önce, kardiak ponksiyon ile amilaz tayini için kan örnekleri alındı ve steril tüplerde muhafaza edildi. Deneklerin pankreas, kolon ve mezokolonları total olarak çıkarıldı ve histopatolojik olarak incelendi. Sonuçlar Mann-Whitney Rank Sum testi ile analiz edildi ve p < 0.05 değerleri istatistiksel olarak anlamlı kabul edildi. Sonuç olarak sıçanda oluşturulan akut pankreatit modelinde kolonun sıklıkla etkilenen segmentinin transvers kolon olduğu ve bunun derecesinin pankreatitin şiddeti ile arttığı kanısına varıldı.
This study was undertaken at the Experimental Surgery Unit of the Aegean University Medical School Hospital. Twenty one Albino rats were divided into three equal groups: Group A as a control, Group B, induced acut pancreatitis and Group B2 induced mild to severe pancreatis by sc injection of 20 $mu$g/kg of cerulein according to various protocols. Two cc of 0.9% NaCl was given by SC injection to group A and they were sacrified by ether anesthesia 24 hours later. 20 $mu$g/kg of cerulein was given by SC infectin to group B1 and in four divided doses over a seven day period to group B2 and they were sacrified by ether anesthesia after 15 days. Two ml blood samples were taken for amilase levels by cardiac puncture prior to killing the rats. Their pancreas, colon and mesocolon were totally excised and examined histopathologically. All results were analyzed by Mann-Whitney Rank Sum test and a p value <0.05 was assigned as significant. Our results showed that the transverse colon was the segment of the colon most affected by acute pancreatitis in rats and that this effect was modified by the severity of pancreatitis.
This study was undertaken at the Experimental Surgery Unit of the Aegean University Medical School Hospital. Twenty one Albino rats were divided into three equal groups: Group A as a control, Group B, induced acut pancreatitis and Group B2 induced mild to severe pancreatis by sc injection of 20 $mu$g/kg of cerulein according to various protocols. Two cc of 0.9% NaCl was given by SC injection to group A and they were sacrified by ether anesthesia 24 hours later. 20 $mu$g/kg of cerulein was given by SC infectin to group B1 and in four divided doses over a seven day period to group B2 and they were sacrified by ether anesthesia after 15 days. Two ml blood samples were taken for amilase levels by cardiac puncture prior to killing the rats. Their pancreas, colon and mesocolon were totally excised and examined histopathologically. All results were analyzed by Mann-Whitney Rank Sum test and a p value <0.05 was assigned as significant. Our results showed that the transverse colon was the segment of the colon most affected by acute pancreatitis in rats and that this effect was modified by the severity of pancreatitis.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Turkish Journal of Gastroenterology
WoS Q Değeri
Scopus Q Değeri
Cilt
9
Sayı
3
