Rapid Fibroblast Cell Culture Characterization with Impedimetric Label-free Heat Shock Protein-47 Biosensor
Küçük Resim Yok
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, for the first time in literature, an impedimetric biosensor technology was developed to determine Heat Shock Protein-47 (HSP-47) for the characterization of primary skin fibroblast cells. Gold nanoparticle electrodes (GNPE) were modified with amino-functionalized graphene oxide layers and anti-HSP-47 were immobilized on this layer. the performance of the biosensor was tested with real samples as cell lysates. the low detection limit (LOD) and the linear detection limit (LOQ) of the biosensor were calculated as 9.47 pg/mL and 3.23 pg/mL, respectively. Linear measurement range was obtained between 10-160 pg/mL HSP-47. the reproducibility of the biosensor was good.
Açıklama
Anahtar Kelimeler
HSP-47, impedance, gold nanoparticle, graphene, chronoimpedance
Kaynak
Electroanalysis
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
32
Sayı
10
