Supercritical carbon dioxide dried double layer laponite XLS and alginate/polyacrylamide construct and immune response
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Churchill Livingstone
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Fabrication of immunocompatible tissue constructs for bone-cartilage defect regeneration is of prime importance. In this study, a double layer hydrogel was successfully synthesized, where alginate/polyacrylamide were formulated to represent cartilage layer (5-10 % (w/w) total polymer ratio) and laponite XLS (2-5-8% (w/w))/ alginate/polyacrylamide formed bone layer. Hydrogels were dried by supercritical CO2 at 100 and 200 bar, 45 degrees C, 5 g/min CO2 flow rate for 2 h. Constructs were treated with collagen, then cellularized and embedded in cell-laden Ge1MA to mimic the cellular micmenvironment. The optimum weight ratio of alginate/polyacrylamide:laponite XLS was 10:5 based on mechanical strength test results. The constructs yielded high porosity (91.50 m(2)/g) and mesoporous structure, owing to the diffusivity of CO2 at 200 bar (0.49 x 10(-7) m(2)/s). Constructs were then treated with collagen to increase cell adhesion and ATDC5 cells were seeded in the cartilage layer, whereas hFOB cells to the bone layer. About 10-15 % higher cell viability was attained. The porous structure of the construct allowed infiltration of macrophages, promoted polarization and positively affected the behavior of macrophages, yielding a decrease in M1 markers, whereas an increase in M2 on day 4. The formulated tissue constructs would be of value in tissue engineering applications.
Açıklama
Anahtar Kelimeler
Laponite, Alginate, Double layer construct, Supercritical CO2, Osteochondral injury, Immune response, Stem-Cell, Nanocomposite Hydrogels, Rheological Behavior, In-Vitro, Scaffold, Cartilage, Polarization, Regeneration, Insights, Biocompatibility
Kaynak
Tissue & Cell
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
74
