Denaturation and renaturation kinetics of DNA in ethyene glycol and glycerol solutions

Nucleıc acids occupy unique position in the biochemical world. Not only are they ivolved in many important reactions, but they carry genetic information, which must be faithfully duplicated so that in can be passed from one cell generation to next and from one organism to next. Nonethless this information must be mutable to produce the variability on which the evolutionary selection process feeds. The DNA must be selectively transcribed so that each cell can synthesize the proteins it needs.Consequently researches with DNA is very important. At present Study, DNA was denatured by heating in saline - citrate buffer which contanied ethylene glycol and glycrol also the kinetics of renaturation was studied. It was found that EG and Glyc reduces the thermal stability of DNA but the ionic strength of EG and Glyc solitions has no effect on the thermal stability. Key words: Renaturation of DNA