MICROBIAL PRODUCTION OF POLY-B-HYDROXYBUTYRATE (PHB) BY BACILLUS MEGATERIUM STRAINS ISOLATED FROM ROOT NODULES OF DIFFERENT FLOWERING PLANTS IN TURKEY

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dc.contributor.authorOmeroglu, Esra Ersoy
dc.contributor.authorOzyildiz, Figen
dc.contributor.authorKaraboz, Ismail
dc.date.accessioned2019-10-27T21:34:11Z
dc.date.available2019-10-27T21:34:11Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this research was the isolation and identification of 5 strains achieved from root nodules of different flowering plants (Vicia sp., Phaeseolus vulgaris, Pisum sativum, Vicia faba), and the determination of poly-beta-hydroxybutyrate (PHB) efficiency of the isolates on different media conditions. Results of our experiments showed that the strains were Bacillus megaterium based on cultural, biochemical and 16S rRNA sequencing methods and VITEK2 system. The amounts and yield of PHB (%) produced by B. megaterium strains were also determined by use of spectrophotometric method. Consequently, the highest yields of PHB were obtained from strain 13/1 (83.82%) on yeast extract mannitol medium (YEM), strain 13/2 (87.50%) with glycin, and strains 13/1 (86.30%) and 2/a (82.03%) with glucose on YEM medium. For further experiments by using these bacteria, determination of less expensive substrates and industrial-scale PHB production will be necessary. For this purpose, B. megaterium strains 13/1, 13/2 and 2/a can be used.en_US
dc.identifier.endpage712en_US
dc.identifier.issn1018-4619
dc.identifier.issn1018-4619en_US
dc.identifier.issue3Aen_US
dc.identifier.startpage704en_US
dc.identifier.urihttps://hdl.handle.net/11454/45589
dc.identifier.volume21en_US
dc.identifier.wosWOS:000302887800006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus megateriumen_US
dc.subjectpoly-beta-hydroxybutyrate (PHB)en_US
dc.subjectnoduleen_US
dc.subject16S rRNAen_US
dc.subjectVITEK2 systemen_US
dc.titleMICROBIAL PRODUCTION OF POLY-B-HYDROXYBUTYRATE (PHB) BY BACILLUS MEGATERIUM STRAINS ISOLATED FROM ROOT NODULES OF DIFFERENT FLOWERING PLANTS IN TURKEYen_US
dc.typeArticleen_US

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