Is losartan a promising agent for the treatment of type 1 diabetes-induced testicular germ cell apoptosis in rats?
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Science and Business Media B.V.
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: Diabetes mellitus (DM) is common metabolic disease that poses a major risk to public health and fertility. Previous studies indicate that DM may cause male infertility by triggering oxidative stress and germ cell apoptosis in the testis. Due to the undesirable effects of known antidiabetic drugs, scientists have begun to investigate the use of alternative drugs to control infertility complications observed in men. In this context, present study aimed to investigate the possible antiapoptotic effect of losartan against DM-induced testicular germ cell apoptosis. Methods and results: Expreimental DM model was induced by intraperitoneal injection of streptozocin (STZ, 55 mg/kg) to 28 rats, which were then randomly assigned to 4 groups; 1 mL saline solution was given to DM + saline group by oral gavage, 5 mg/kg/day oral losartan was given to DM + low-dose losartan, 20 mg/kg/day oral losartan was given to DM + mid-dose losartan and, 80 mg/kg/day oral losartan was given to DM + high-dose losartan group for 4 weeks. Bax, Bcl-2 and cleaved-Caspase 3 immunoexpression, terminal-deoxynucleotidyl transferase dutp nick end labeling (TUNEL), Annexin-V and Real Time PCR analyses performed to evaluate antiapoptotic effects of losartan on diabetic rats’ testis. In addition, biochemical analyzes carried out to evaluate change in oxidative stress. Conclusion: The results showed that losartan may have dose-related antiapoptotic effects on rats’ testis via decreasing oxidative stress. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.
Açıklama
Anahtar Kelimeler
Apoptosis, Diabetes, Losartan, Oxidative stress, Testis, caspase 3, losartan, losartan potassium, malonaldehyde, protein Bax, protein bcl 2, superoxide dismutase, testosterone, losartan, streptozocin, animal experiment, animal model, animal tissue, antiapoptotic activity, antioxidant activity, Article, controlled study, dose response, drug dose comparison, drug megadose, germ cell, insulin dependent diabetes mellitus, Leydig cell, lipid peroxidation, low drug dose, male, male infertility, mRNA expression level, nonhuman, oxidative stress, protein expression, rat, seminiferous tubule, sperm count, spermatocyte, streptozotocin-induced diabetes mellitus, testicular germ cell, testis, animal, apoptosis, experimental diabetes mellitus, metabolism, Animals, Apoptosis, Diabetes Mellitus, Experimental, Diabetes Mellitus, Type 1, Germ Cells, Losartan, Male, Oxidative Stress, Rats, Streptozocin, Testis
Kaynak
Molecular Biology Reports
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
50
Sayı
3
