A Novel Chronoimpedimetric Cytosine Modified Molecularly Imprinted Sensor for Label-Free Detection of 8-Hydroxydeoxyguanosine in Urine Samples
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Electrochemical Soc Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
First time in the literature, we imprinted 8-hydroxyguanosine (8OHG) to engineer an impedimetric sensor on a polymer from three different main non-covalent structures. Considering the chemical structure of the molecule and its potential non-covalent bonding behaviors, we designed a special electrode with molecular imprinting technology (MIT). Therefore, 8OHG, which serves as an important biomarker of oxidative stress, was imprinted on an electrode to form an 8OHG sensor. In this imprinting method, firstly, a gold electrode was modified with Cytosine-1yl-acetic acid (CAA) to increase selectivity and form DNA hydrogen bond-like structures. Afterward, pyrrole and aminophenyl boronic acid monomers were polymerized from three different points by electropolymerization, and a selective and sensitive sensor technology was developed. 8OHG measurement was carried out impedimetrically in six minutes (R-2 value in the range of 500-10000 pg ml(-1) is 0.9928 +/- 0.006). LOD and LOQ was calculated 155.8 pg ml(-1) and 472 pg ml(-1), respectively. In conclusion, a sensitive, low-cost, fast and innovative technique with higher selectivity has been introduced. We believe that novel imprinting techniques will provide the important potential for MIP techniques for medical diagnostics.
Açıklama
Anahtar Kelimeler
8-hydroxyguanosine, impedance, sensor, molecular imprinting, chronoimpedance, oxidant stress, Oxidative Stress, 8-Hydroxy-2'-Deoxyguanosine, Chromatography, Dna
Kaynak
Journal Of The Electrochemical Society
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
169
Sayı
10
