Sol-Gel/Chitosan/Gold Nanoparticle-Modified Electrode in Mediated Bacterial Biosensor
Küçük Resim Yok
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A mediated whole cell biosensor was designed and characterized using glucose as the substrate. Gluconobacter oxydans cells were immobilized on the graphite electrode via sol-gel (tetraethyl orthosilicate)/chitosan hybrid composite modified with gold nanoparticles (TEOS/CHIT/AuNPs/G. oxydans). A soluble mediator, potassium hexacyanoferrate (III), was used for the amperometric detection of the respiratory activity of the intact bacterial cells. After optimization of preparation conditions of the TEOS/CHIT/AuNPs/G. oxydans biosensor, characterization studies have been carried out. The linear range for glucose was found to be 0. 5-2. 0 mM with a response time of 80 s. Moreover, repeatability and electrode-to-electrode reproducibility of the system were investigated and relative standard deviations were calculated as 1. 7% and 2. 2% for 1. 0 mM glucose (n = 5). Finally, the proposed system was applied to ethanol and glucose detection in real samples and the results were compared with a reference method. © 2011 Springer Science+Business Media, LLC.
Açıklama
Anahtar Kelimeler
Biosensor, Chitosan, Gold nanoparticles, Mediated microbial sensor, Sol-gel
Kaynak
Food Analytical Methods
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
5
Sayı
2
