Salmonella enterica kökenlerinde azalmış siprofloksasin duyarlılığı ve nalidiksik asit tarama testi
Küçük Resim Yok
Tarih
2006
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
İnvazif Salmonella infeksiyonlarında florokinolonların ilk seçilecek ilaçlar olmasına karşın son zamanlarda bir çok ülkeden azalmış duyarlılık ve buna bağlı tedavi başarısızlıkları bildirilmektedir. Bu çalışmanın amacı, Salmonella enterica kökenlerinde azalmış siprofloksasin duyarlılığını araştırmaktır. Çalışmada 1997-2005 yılları arasında soyutlanan 133 S. enterica kökeni retrospektif olarak incelendi. Nalidiksik asit ve siprofloksa-sin duyarlılıkları Clinical and Laboratory Standards Institute (CLSI) önerileri doğrultusunda disk difüzyon, siprofloksasin MİK değerleri ise E-test yöntemi ile belirlendi. Azalmış siprofloksasin duyarlılığı, siprofloksasin MİK ? 1 µg/ml - ? 0.125 µg/ml olması olarak tanımlandı. İncelenen kökenlerin siprofloksasin MİK değerleri 0.004-0.25 µg/ml. arasında bulundu. Kökenlerin 22'si nalidiksik aside dirençli, 111'i duyarlı idi. Duyarlı 111 kökenin siprofloksasin MİK'leri ? 0.064 µg/ml, dirençli 22 kökenin ise 0.125-0.25 µg/ml arasındaydı. Siprofloksasin MİK'leri ? 0.064 µg/ml olan kökenlerin disk difüzyon yöntemindeki nalidiksik asit inhibisyon zon çapları ? 33 mm bulundu. Siprofloksasine azalmış duyarlılık görülen kökenlerin zon çapları ise 25-32 mm arasında idi. Soyutlanan S. enterica kökenleri arasında %16.5 oranında azalmış siprofloksasasin duyarlılılığı belirlendi. Disk difüzyon yöntemi ile nalidiksik asit direnci araştırmanın azalmış siprofloksasin duyarlılığı olan kökenleri belirlemede yararlı olduğu görüldü.
Although the fluoroquinolones are the drugs of choice for invasive Salmonella infections, recently reduced ciprofloxacin susceptibility and therapeutic failures have been reported from many countries. The purpose of this study was to determine reduced ciprofloxacin susceptibity among Salmonella enterica strains isolated in the routine laboratory. A total of 133 Salmonella enterica isolates collected between 1997-2005 were studied retrospectively. Nalidixic acid and ciprofloxacin susceptibilities were investigated by disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines, also ciprofloxacin MIC values were determined by E-test. Reduced ciprofloxacin susceptibility was described as MIC values ? 1 µg/ml - ? 0.125 µg/ml. Ciprofloxacin MIC values of all 133 isolates were between 0.004-0.25 µg/ml. Twenty two isolates were classified as nalidixic acid resistant while the rest 111 were evaluated as sensitive. Ciprofloxacin MIC values of these 111 strains were ? 0.064 µg/ml and 22 isolates had MIC's between 0.125-0.25 µg/ml. Isolates for which the ciprofloxacin MICs were ? 0.064 µg/ml had inhibition zone diameters ? 33 mm for nalidixic acid whereas isolates showing decreased ciprofloxacin susceptibility had zone diameters of 25-32 mm. Among the S. enterica strains isolated in the laboratory 16.5% reduced ciprofloxacin susceptibility and nalidixic acid resistance were detected. To determine the reduced ciprofloxacin susceptibility, screening nalidixic acid resistance by disk diffusion was observed to be helpful.
Although the fluoroquinolones are the drugs of choice for invasive Salmonella infections, recently reduced ciprofloxacin susceptibility and therapeutic failures have been reported from many countries. The purpose of this study was to determine reduced ciprofloxacin susceptibity among Salmonella enterica strains isolated in the routine laboratory. A total of 133 Salmonella enterica isolates collected between 1997-2005 were studied retrospectively. Nalidixic acid and ciprofloxacin susceptibilities were investigated by disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines, also ciprofloxacin MIC values were determined by E-test. Reduced ciprofloxacin susceptibility was described as MIC values ? 1 µg/ml - ? 0.125 µg/ml. Ciprofloxacin MIC values of all 133 isolates were between 0.004-0.25 µg/ml. Twenty two isolates were classified as nalidixic acid resistant while the rest 111 were evaluated as sensitive. Ciprofloxacin MIC values of these 111 strains were ? 0.064 µg/ml and 22 isolates had MIC's between 0.125-0.25 µg/ml. Isolates for which the ciprofloxacin MICs were ? 0.064 µg/ml had inhibition zone diameters ? 33 mm for nalidixic acid whereas isolates showing decreased ciprofloxacin susceptibility had zone diameters of 25-32 mm. Among the S. enterica strains isolated in the laboratory 16.5% reduced ciprofloxacin susceptibility and nalidixic acid resistance were detected. To determine the reduced ciprofloxacin susceptibility, screening nalidixic acid resistance by disk diffusion was observed to be helpful.
Açıklama
Anahtar Kelimeler
Enfeksiyon Hastalıkları
Kaynak
İnfeksiyon Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
20
Sayı
2
