Inherent non-linearities in immobilized enzyme assays and possibilities for selectivity in enzyme immobilization
Küçük Resim Yok
Tarih
1983
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kluwer Academic Publishers
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Specific activities of immobilized ?-amylases toward native amylose and soluble starch have been determined at various immobilized enzyme concentrations. The resultant 'activity versus enzyme concentration' curves are shown to display inherently non-linear behaviour arising from the polymeric character of the substrate. Based on the conclusions drawn from the nature of the non-linearities it is hypothesized that the enzyme molecules tend to concentrate more on the surface of the carrier than inside the pores as the reactivities of the components in the coupling reaction are increased. © 1983 Science and Technology Letters.
Açıklama
Anahtar Kelimeler
Kaynak
Biotechnology Letters
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
5
Sayı
5
