Peroxiredoxins and Hypoxia-Inducible Factor-1 alpha in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Celiac disease (CD) is an autoimmune enteropathy. Peroxiredoxins (PRDXs) are powerful antioxidant enzymes having an important role in significant cellular pathways including cell survival, apoptosis, and inflammation. This study aimed at investigating the expression levels of all PRDX isoforms (1-6) and their possible relationships with a transcription factor, HIF-1 alpha, in the small intestinal tissue samples of pediatric CD patients. The study groups consisted of first-diagnosed CD patients (n = 7) and non-CD patients with functional gastrointestinal tract disorders as the controls (n = 7). The PRDXs and HIF-1 alpha expression levels were determined by using real-time PCR and Western blotting in duodenal biopsy samples. It was observed that the mRNA and protein expression levels of PRDX 5 were significantly higher in the CD patients, whereas the PRDX 1, -2, and -4 expressions were decreased in each case compared to the control group. No significant differences were detected in the PRDX 3 and PRDX 6 expressions. The expression of HIF-1 alpha was also significantly elevated in CD patients. These findings indicate, for the first time, that PRDXs, particularly PRDX 5, may play a significant role in the pathogenesis of CD. Furthermore, our results suggest that HIF-1 alpha may upregulate PRDX-5 transcription in the duodenal tissue of CD.
Açıklama
Anahtar Kelimeler
celiac disease small intestine, inflammation, antioxidant, peroxiredoxin, hypoxia-inducible factor-1alpha, Colorectal-Cancer Cells, Oxidative Stress, Factor-I, Gliadin Peptides, Messenger-Rna, Inflammation, Expression, Children, Protein, Hif-1
Kaynak
Current Issues In Molecular Biology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
45
Sayı
2
