Katı Kültür Fermantasyon Tekniği ile Streptomyces sp. TEM25'ten Ksilanaz Üretimi
Küçük Resim Yok
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, endüstriyel ve biyoteknolojik alanda geniş yer tutan ksilanaz enzimi üretimi için yeni gen kaynakları araştırılmış ve üretim koşulları optimize edilerek enzim üretim verimi arttırılması amaçlanmıştır. Bu nedenle, katı kültür ve batık kültür fermantasyonu teknikleri karşılaştırılmış, fermantasyon için başlangıç inokülasyon şekli ve miktarı optimize edilmiş ve nemlendirme oranının enzim üretim verimine etkisinin belirlenmesi hedeflenmiştir. Topraktan izole edilen 49 Aktimoniset izolatı içinden, en iyi ksilanaz üretici olarak belirlenen Streptomyces TEM 25 straininden ksilanaz üretiminin en iyi buğday kepeği kullanılarak yapılan katı kültür fermantasyonunda gerçekleştiği tespit edilmiştir. Katı kültür fermantasyon koşulları optimize edilerek ksilanaz enzim aktivitesi yaklaşık 1,5 kat arttırılmış ve %66,6 nemlendirme koşullarında 45,85 ±1.22 U/g kepek enzim aktivitesine ulaşıldığı belirlenmiştir. Amonyum sülfat çöktürme yöntemi ile kısmi saflaştırılan enzimin spesifik aktivitesi 5 kat artırılmıştır.
In this study, new gene sources for the production of xylanase enzyme, which occupies a large area in industrial and biotechnological field, were investigated and optimization of production conditions was aimed to increase enzyme production efficiency. For this reason, the techniques of solid state fermentation and submerged fermentation were compared, the initial inoculation type and amount were optimized and the effect of humidification rate on the efficiency of enzyme production was determined. From 49 Actinomycetes strains isolated from soil, it was determined that xylanase production from Streptomyces TEM 25, which was identified as the best xylanase producer, was best performed in solid culture fermentation using wheat bran. After the optimization of the solid state fermentation conditions, xylanase activity was increased 1.5 fold to 45.85 ±1.22 U/g wheat bran under 66.6% moistened conditions. The specific activity of the partially purified enzyme was increased 5-fold by the ammonium sulfate precipitation method.
In this study, new gene sources for the production of xylanase enzyme, which occupies a large area in industrial and biotechnological field, were investigated and optimization of production conditions was aimed to increase enzyme production efficiency. For this reason, the techniques of solid state fermentation and submerged fermentation were compared, the initial inoculation type and amount were optimized and the effect of humidification rate on the efficiency of enzyme production was determined. From 49 Actinomycetes strains isolated from soil, it was determined that xylanase production from Streptomyces TEM 25, which was identified as the best xylanase producer, was best performed in solid culture fermentation using wheat bran. After the optimization of the solid state fermentation conditions, xylanase activity was increased 1.5 fold to 45.85 ±1.22 U/g wheat bran under 66.6% moistened conditions. The specific activity of the partially purified enzyme was increased 5-fold by the ammonium sulfate precipitation method.
Açıklama
Anahtar Kelimeler
Mühendislik, Ziraat
Kaynak
Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
34
Sayı
2
