Complications in rats created by human bile and gallstones

Background/aims: In this study human bile and stones were placed into the abdominal cavities of rats to observe if complications such as adhesions, abscesses and fistulas would occur. Methods: Stones and bile samples used were taken under sterile conditions from a patient who did not have any previous gallbladder disease such as acute attack or perforation. Results: During the eight-week study period, the following observations were made: adhesion in the bile and stone implant group 84% (p<0.05); adhesion in the bile only implant group: 50%, adhesion in the stone only implant group: 59%. No other pathological findings such as abscesses or fistula formation were found. Conclusion: The utmost care must be taken not to perforate the gallbladder during laparoscopic cholecystectomy.