Diffusion Characteristics of Chitosan-Entrapped Microsomal Udp-Glucuronyl Transferase Gel Beads
Küçük Resim Yok
Tarih
1995
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Rabbit hepatic microsomal UDP-glucuronyl transferase (EC 2.4.1.17) was immobilized by entrapment in chitosan which is an ionotropic gelation agent and the resulting preparation was used as a biocatalyst for the glucuronidation of 1-naphthol in order to study the drug metabolism in vitro and obtaining artificial liver support (detoxification). In this study, depending on the porous structure of the chitosan/UDPGT gel beads, mainly the diffusion characteristics of 1-naphthol from chitosan gel beads in well stirred solutions were investigated, and the values of the effective diffusion coefficients (D-e) indicate that UDP-glucuronyl transferase in chitosan beads has no additional diffusion barrier. Using the data, the capacity of the chitosan-gel as an immobilization matrix for microsomal UDP-glucuronyl transferase is discussed from the point of view of diffusion characteristics.
Açıklama
Anahtar Kelimeler
DIFFUSION, CHITOSAN-ENTRAPPED UDP-GLUCURONYL TRANSFERASE, IMMOBILIZATION
Kaynak
Biochimica Et Biophysica Acta-General Subjects
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
1244
Sayı
02.Mar
