Investigation of the interaction between fat body and ovary development pupal transformation in silkworm, Bombyx mori
Küçük Resim Yok
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Böcek yağ dokusu, lipit, karbohidrat, aminoasit ve nitrojen metabolizmalarına katılan ve protein sentezinde rol alan en büyük biyosentetik depo organıdır. Bunun yanı sıra, vitellogenin gibi yumurta depo proteinlerinin öncülerini sentezleyerek aktif olarak vitellogenezde ve ovaryum gelişiminde rol alır. Bombyx mori’de (L.)’te metamorfoz süresince, yağ doku, hemolenf ve ovaryum arasında meydana gelen sürekli madde değişimi ekdisteroidler ve juvenile hormonun kontrolü altında gerçekleşir. Elektroforetik olarak belirlenen temel hemolenf proteinleri şu şekilde gruplandırılmıştır: depo proteinleri (72 kDa ve 76 kDa), 30K polipeptidleri, ApoLp-I (230- 250 kDa), yumurta spesifik proteini (/2 kDa ve 64 kDa) ve vitellogenin (178 kDa). Çalışmamızın sonucunda metamorfoz süresince yağ doku proteinlerinin nitelik ve niceliğindeki değişimlerin ovaryum ve yumurta gelişimi üzerinde doğrudan etkiye sahip olduğu sonucuna ulaşılmıştır.
The insect fat body is the major biosynthetic and storage organ involved in lipid, carbohydrate, amino acid and nitrogen metabolism and protein synthesis. It also actively participates in vitellogenesis and ovary development, synthesizing the soluble precursor for yolk, i.e., vitellogenin in stage specific manner. The continual substance exchange among fat body, hemolymph and ovary is controlled by hormones, ecdysteroids and juvenile hormone, during metamorphosis of Bombyx mori (L.). The aim of the present study was to clarify the interactions between major proteins of fat body and hemolymph and their effect on ovarian development under hormonal factors during pupal-adult transformation. Detected fat body, hemolymph and ovary proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE): storage proteins (72 kDa and 76 kDa), 30 kDa proteins, ApoLp-I (230-250 kDa), egg specific protein (72 kDa and 64 kDa) and vitellogenins (178 kDa). Our results suggest that changes in the well-defined and characterized protein fractions quality and quantity of the fat body had a direct effect under hormonal factors on the ovary and egg development during metamorphosis.
The insect fat body is the major biosynthetic and storage organ involved in lipid, carbohydrate, amino acid and nitrogen metabolism and protein synthesis. It also actively participates in vitellogenesis and ovary development, synthesizing the soluble precursor for yolk, i.e., vitellogenin in stage specific manner. The continual substance exchange among fat body, hemolymph and ovary is controlled by hormones, ecdysteroids and juvenile hormone, during metamorphosis of Bombyx mori (L.). The aim of the present study was to clarify the interactions between major proteins of fat body and hemolymph and their effect on ovarian development under hormonal factors during pupal-adult transformation. Detected fat body, hemolymph and ovary proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE): storage proteins (72 kDa and 76 kDa), 30 kDa proteins, ApoLp-I (230-250 kDa), egg specific protein (72 kDa and 64 kDa) and vitellogenins (178 kDa). Our results suggest that changes in the well-defined and characterized protein fractions quality and quantity of the fat body had a direct effect under hormonal factors on the ovary and egg development during metamorphosis.
Açıklama
Anahtar Kelimeler
Biyoloji
Kaynak
IUFS Journal of Biology
WoS Q Değeri
Scopus Q Değeri
Cilt
72
Sayı
2
