LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION
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Tarih
2021
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Laccase was partially purified from mushroom compost with cheap and simple purification methods. Partially purified laccase was immobilized onto Amberlite XAD-7 resin. Immobilization conditions and textile dye (Direct Green B) decolorization of immobilized enzyme were examined. Laccase was partially purified from mushroom compost with 3.22 fold. Immobilization time for laccase was 30 minutes with 97% efficiency. The immobilization yield based on the activity was calculated by about 90%. The immobilization yield was calculated as nearly 95% based on the measurement of protein amount at each stage. Immobilized enzyme preserved its initial activity with an 84% rate even after its tenth use. The free enzyme lost its activity immediately when it was incubated with the dye solution under its optimal conditions (pH 3.0 and 65°C). On the contrary, the immobilized enzyme maintained its initial activity with a 53% rate when incubated with dye solution under its optimal conditions (pH 3.5 and 65°C).
Açıklama
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Kaynak
Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online)
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Cilt
10
Sayı
1
