İnsan meme kanserlerinde Ki-67'nin immunohistışimik yöntemlerle gösterilmesi ve bunun prognozla ilişkisi
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2000
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Ege Üniversitesi
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info:eu-repo/semantics/openAccess
Özet
Meme kanserlerinde, tümör proliferatif fraksiyonun (TPF) prognostik önemi olduğu gösterilmiştir. Son çalışmalarda hücre siklusunun istirahat halindeki fazı dışındaki tüm fazları boyunca pozitif bulunan, nükleer bir antijen olan Ki-67’nin meme tümörlerindeki prognostik önemi yoğun olarak araştırılmaktadır. Bu çalışmada prognoza ilişkin iki morfolojik parametre olarak Ki-67 işaretlemesi ve nükleoler organize edici bölge (AgNOR) boyanması, 35 meme karsinomu olgusunda incelenmiş, sonuçlar birbiri ve tümör boyutu, histolojik derece ve aksiller lenf nodülü tutulumu ile karşılaştırılmıştır. Ki-67 immunreaktivitesinin değerlendirilmesi amacı ile dondurulmuş doku kesitlerinde alkalen fosfataz anti-alkalen fosfataz yöntemiyle immünperoksidaz boyanması uygulanmış ve pozitif boyanan hücre oranının saptanması amacıyla rastgele seçilen en az 1000 hücre sayılmıştır. Ag-NOR sayısının saptanması için kolloid gümüşle boyanan kesitlerde, rastgele seçilmiş en az 100 hücre sayılmış ve her tümör için ortalama bir skor belirlenmiştir. Tüm olgularda ortalama TPF değerleri, Ki-67 için %9.9, Ag-NOR için %7.4 olarak saptanmış, bu değerler birbiri ile kıyaslanmış, sonuçlar istatiksel olarak anlamlı bulunmuştur (p<0.01). Tümör boyutu, histolojik derecesi ve metastatik lenf nodülü sayısı ile Ki-67 immünreaktivitesi ve ortalama NOR sayısı arasında belirgin bir korelasyon görülmemiştir. Bu bulgular , Ki-67 ve Ag-NOR boyamalarının TPF değerlerini belirlemede benzer iki yöntem olduğunu göstermektedir, ancak mitotik indeksin Ki-67 ile saptanması Ag-NOR boyanmasına kıyasla daha güvenilir bir yöntem olarak ortaya çıkmaktadır.
Tumor proliferative fraction (TPF) has been shown to have prognostic significance in breast cancer. Recent studies have begun exploring the nuclear antigen Ki-67 which is present in all phases of the cell cycle except the resting phase. The current study was performed on 35 cases of breast carcinoma using Ki-67 labeling and argyrophilic nucleolar organizer region (Ag-NOR) staining as the two morphometric parameters associated with prognosis and the results were correlated with each other, plus the tumor size, histologic grade and axillary lymph node status. Immunoperoxidase staining by the alkaline phosphatase anti-alkaline phosphatase method was performed on the frozen tissue sections for the evaluation of Ki-67 immunoreactivity and a minimum of 1000 randomly selected cells were calculated for the determination of the percentage of stained cells. The mean number of NOR was counted in a minimum of 100 randomly selected cells on colloid silver-stained sections and an average score was determinated for each tumor. The mean TPF values for all cases were 9.9% by Ki-67 and 7.4% by Ag-NOR staining, which correlated significantly at p<0.01. No significant positive correlation was found between the tumor size, histologic grading of the tumor, and the number of lymph node metases with Ki-67 immunoreactivity, and mean number of NOR. These data suggest that both Ki-67 and Ag-NOR stainings are two related methods in the determination of TPF values, but assesing the mitotic index with Ki-67 is a more reliable method when compared with Ag-NOR staining.
Tumor proliferative fraction (TPF) has been shown to have prognostic significance in breast cancer. Recent studies have begun exploring the nuclear antigen Ki-67 which is present in all phases of the cell cycle except the resting phase. The current study was performed on 35 cases of breast carcinoma using Ki-67 labeling and argyrophilic nucleolar organizer region (Ag-NOR) staining as the two morphometric parameters associated with prognosis and the results were correlated with each other, plus the tumor size, histologic grade and axillary lymph node status. Immunoperoxidase staining by the alkaline phosphatase anti-alkaline phosphatase method was performed on the frozen tissue sections for the evaluation of Ki-67 immunoreactivity and a minimum of 1000 randomly selected cells were calculated for the determination of the percentage of stained cells. The mean number of NOR was counted in a minimum of 100 randomly selected cells on colloid silver-stained sections and an average score was determinated for each tumor. The mean TPF values for all cases were 9.9% by Ki-67 and 7.4% by Ag-NOR staining, which correlated significantly at p<0.01. No significant positive correlation was found between the tumor size, histologic grading of the tumor, and the number of lymph node metases with Ki-67 immunoreactivity, and mean number of NOR. These data suggest that both Ki-67 and Ag-NOR stainings are two related methods in the determination of TPF values, but assesing the mitotic index with Ki-67 is a more reliable method when compared with Ag-NOR staining.
Açıklama
Anahtar Kelimeler
Meme Kanseri, Ki-67, Ag-NOR, Breast Cancer, Ki-67, Ag-NOR
