İnulinaz enziminin üretimi ve üretim kinetiklerinin belirlenmesi
Küçük Resim Yok
Tarih
1988
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
- 73 - 6. ÖZET Endüstriyel uygulamalarda biyolojik katalizör olan enzimlerin kullanımının artmasına paralel olarak endüst riyel enzim üretimi de artış göstermiştir. Endüstriyel - enzim üretiminde mikroorganizmalar sahip oldukları potansiyel nedeniyle bitk
75 7. SUMMARY Enzymes are biological catalysts which have found increasing applications in the industry. Production of enzymes have thus paralleled this demand. Microorganis ms have been preferred againts plant and animal sources for enzyme production due to their high productivity potentials in microbial enzyme production. The growth conditions and the parameters which effect the process need to be optimized which ultimately determines the process economy. The raw material greatly contributes to the final product cost, therefore alternative raw materials such as Jerusalem artichoke, dahlia, chicory have been suggested as alternative substrates for such processes. In common, these substrates have a very high inulin content. Inulin is a B -2,1 linked fructose polymer with a glucose molecule at the end of this polymer. However, in biotechnological processes this polymer needs to be hydrolized into free sugars in order to be used as an energy and carbon sources. Similarly inulin can also be used as an alternative natural sweetner provi ded that it is hydrolized and concentrated. For the selection of inulinase producing organizm, 31 A.niger strains have been screened which were previously identified in our department and TÜBİTAK for aflatoxin production. Growth media composition was optimized by supplementing with nitrogen, phospho- rousmagnesium and yeast extract in appropiate quanti- Q ties. Spor inoculum at 10 /ml gave better results than mycelium inoculum and an enzyme activity of 36 U/ml was achieved in 96 h of incubation. These results com pared favourobly with the results reported in the literature. Presence of particulate material or76 - phenolic compounds from the Jerusalem artichoke skins did not significantly effect the enzyme production. For the determitation of inulinase activity a tem perature of 50°C and pH 5 was found to be optimum. However enzyme stability measured for one hour without the substrate at various temperatures showed that 77 % of the activity was retained at 60°C and 33 % retained at 65°C. For these temperatures pH stability was found to be between 5-6. Investigations using A.niger A42 strain showedthat it was possible to obtain an enzyme preparation with 50 U/ml activity against inulin within 120 h of incuba tion. Compared to previously reported data, a 50 % reduc tion in process time was possible with simultaneous achievement of the same level of activity. In near future, It is projected that inulinase enzyme will find a wider application both in the food industry and in other biotechnological processes.
75 7. SUMMARY Enzymes are biological catalysts which have found increasing applications in the industry. Production of enzymes have thus paralleled this demand. Microorganis ms have been preferred againts plant and animal sources for enzyme production due to their high productivity potentials in microbial enzyme production. The growth conditions and the parameters which effect the process need to be optimized which ultimately determines the process economy. The raw material greatly contributes to the final product cost, therefore alternative raw materials such as Jerusalem artichoke, dahlia, chicory have been suggested as alternative substrates for such processes. In common, these substrates have a very high inulin content. Inulin is a B -2,1 linked fructose polymer with a glucose molecule at the end of this polymer. However, in biotechnological processes this polymer needs to be hydrolized into free sugars in order to be used as an energy and carbon sources. Similarly inulin can also be used as an alternative natural sweetner provi ded that it is hydrolized and concentrated. For the selection of inulinase producing organizm, 31 A.niger strains have been screened which were previously identified in our department and TÜBİTAK for aflatoxin production. Growth media composition was optimized by supplementing with nitrogen, phospho- rousmagnesium and yeast extract in appropiate quanti- Q ties. Spor inoculum at 10 /ml gave better results than mycelium inoculum and an enzyme activity of 36 U/ml was achieved in 96 h of incubation. These results com pared favourobly with the results reported in the literature. Presence of particulate material or76 - phenolic compounds from the Jerusalem artichoke skins did not significantly effect the enzyme production. For the determitation of inulinase activity a tem perature of 50°C and pH 5 was found to be optimum. However enzyme stability measured for one hour without the substrate at various temperatures showed that 77 % of the activity was retained at 60°C and 33 % retained at 65°C. For these temperatures pH stability was found to be between 5-6. Investigations using A.niger A42 strain showedthat it was possible to obtain an enzyme preparation with 50 U/ml activity against inulin within 120 h of incuba tion. Compared to previously reported data, a 50 % reduc tion in process time was possible with simultaneous achievement of the same level of activity. In near future, It is projected that inulinase enzyme will find a wider application both in the food industry and in other biotechnological processes.
Açıklama
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Anahtar Kelimeler
Gıda Mühendisliği, Food Engineering