Sea urchin micronucleus assay for determination of genotoxic effects of bisphenol a

Bisphenol a (BPA) have generated concern because of to their high production and widespread use. BPA is used to make polycarbonate, epoxy resin production and specialty resins and in the manufacture of flame retardants. It is well-known that a wide variety of man-made chemicals in the environment is capable of adversely affecting aquatic organisms. Damage caused on the DNA by genotoxic pollutants is the first consequence occurring in the aquatic organisms. Micronuclei tests is a system of mutagenicity testing used for determining the pollution and chemicals causing changes in DNA fragments such as micronuclei. Sea urchins are to be good applicants for being selected as marine target macroinvertebrates and useful test species in ecotoxicology. For this purpose, the experiment was carried out using a sea urchin Paracentrotus lividus embryos. The sea urchin embryos were exposed to increasing concentrations of BPA (12.5, 25, 50, 75 and 100 µg-BPA/L) under static conditions in the laboratory. According to the results of the study, it is revealed that all concentrations of BPA increases the genotoxic damage by the means of increasing micronucleus frequency. © 2016 PSP.