Dolaşımdaki belirli miRNA'ların pulmoner arteriyel hipertansiyon gelişimi ve teşhisinde önemi
Yükleniyor...
Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Pulmoner arter hipertansiyonu (PAH), sağ ventrikül hipertrofisine ve kalp yetmezliğine yol açan pulmoner vaskülaritenin maladaptasyonu ile karakterizedir. MikroRNA'lar (miRNA), gen ekspresyonunu transkripsiyonel veya post-transkripsiyonel seviyede negatif bir şekilde düzenleyen, yaklaşık 22 nükleotitten oluşan küçük kodlanmayan RNA’lardır. miRNA'lar, viral enfeksiyon, kanser, kardiyovasküler hastalıklar ve PAH gibi birçok hastalığın düzenlenmesinde çok önemli bir rol oynamaktadır. Bu çalışmada, hafiften şiddetliye PAH hastalarında ve sağlıklı kontrol gruplarında, sekiz insan plazma miRNA (hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p) ekspresyon paternini araştırmayı amaçladık. PAH hastalarının (n: 50) ve sağlıklı bireylerin (n: 30) plazmasından miRNA izolsyon kiti kullanılarak miRNA eksrakte edildi. Allin-One First Strand cDNA Sentez Kiti kullanılarak cDNA sentezlendi. qRT-PCR ile insan plazmasındaki hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p ekspresyonları analiz edildi. Bulgularımıza göre PAH plazma örneklerindeki hsa-miR-21-3p ve hsa-miR-143-3p ekspresyonları sırasıyla 4 ve 2 kat şeklinde azaldı. Hasta ve kontrol gruplarındaki hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p ekspresyon seviyelerinde belirgin bir değişiklik saptanmadı. PAH’ın erken tanısında periferal plazmadaki hsa-miR-21-3p ve hsa-miR-143-3p potansiyel biyobelirteç olarak nitelendirilebilir.
Pulmonery arteriyel hypertension (PAH) is characterized by maladaptation of pulmonery vascularute which is leading to right ventricular hypertrophy and hearth failure. MicroRNAs (miRNA) are the small non-coding RNAs which consist of ~22 nucleotides, regulate the gene expression negatively at the transcriptional or post-transcriptional level. miRNAs play a crucial role in the regulation of many diseases such as viral infection, cancer, cardiovascular diseases,and PAH. In this study, we aimed to investigate the expression pattern of eight human plasma (hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p) miRNAs in mild-to-severe PAH patients and healty controls. miRNAs were extracted from peripheral plasma of the PAH patients (n:50) and healty individuals (n:30) by using the miRNA Isolation Kit. cDNA was synthesised using Allin-One First strand cDNA Synthesis Kit. Expression of the human plasma hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p miRNAs were analysed by qRT-PCR. According to our results, in PAH patients hsa-miR-21-3p and hsa-miR-143-3p expressions levels were decreased 4 and 2 times, respectively. No significant changes were detected in hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p expression levels between patient and control group. In the early diagnosis of PAH, hsa-miR-21-3p and hsa-miR-143-3p in peripheral plasma can be considered as potential biomarkers.
Pulmonery arteriyel hypertension (PAH) is characterized by maladaptation of pulmonery vascularute which is leading to right ventricular hypertrophy and hearth failure. MicroRNAs (miRNA) are the small non-coding RNAs which consist of ~22 nucleotides, regulate the gene expression negatively at the transcriptional or post-transcriptional level. miRNAs play a crucial role in the regulation of many diseases such as viral infection, cancer, cardiovascular diseases,and PAH. In this study, we aimed to investigate the expression pattern of eight human plasma (hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p) miRNAs in mild-to-severe PAH patients and healty controls. miRNAs were extracted from peripheral plasma of the PAH patients (n:50) and healty individuals (n:30) by using the miRNA Isolation Kit. cDNA was synthesised using Allin-One First strand cDNA Synthesis Kit. Expression of the human plasma hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p miRNAs were analysed by qRT-PCR. According to our results, in PAH patients hsa-miR-21-3p and hsa-miR-143-3p expressions levels were decreased 4 and 2 times, respectively. No significant changes were detected in hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p expression levels between patient and control group. In the early diagnosis of PAH, hsa-miR-21-3p and hsa-miR-143-3p in peripheral plasma can be considered as potential biomarkers.
Açıklama
Anahtar Kelimeler
miRNA, hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p, PAH, Biyobelirteç, Biyomarkır, miRNA, hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p, PAH, Biomarker
