Detection of interaction between metal complex indicator and DNA by using electrochemical biosensor
Küçük Resim Yok
Tarih
1999
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
There has been extensive research on binding of transition metal complexes to DNA via electrostatic and hydrophobic interactions. Most indicator based electrochemical DNA biosensors have used cationic metal complexes that interact in a different way with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Described here are the electrochemical parameters for a mixed-ligand complex, [Co(phen)(3)(3+)] (phen: 1,10-phenanthroline), on binding to DNA. The milimolar quantities of [Co(phen)(3)(3+)], which associates reversibly with immobilized calf thymus DNA was detected by using dsDNA-modified carbon paste electrode (dsDNA-modified CPE), ssDNA-modified carbon paste electrode (ssDNA-modified CPE) and bare carbon paste electrode (bare CPE), voltammetrically and the decreased peak currents were observed, respectively. The extend of hybridization between the complementary sequences is determined by the enhancement of the voltammetric peak of the [Co(phen)(3)(3+)] indicator. Numerous factors affecting the DNA irmnobilization and indicator were investigated. Experiments were also performed at various salt concentrations and the optimum salt concentration was determined. The difference between the peak currents of denaturated calf thymus DNA (ssDNA)-modified CPE and dsDNA-modified CPE was also observed. These results demonstrated the use of the electroactive hybridization indicator, [Co(phen)(3)(3+)] for DNA biosensers.
Açıklama
Anahtar Kelimeler
DNA biosensors, cobalt phenanthroline, DNA, hybridization
Kaynak
Electroanalysis
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
11
Sayı
18
