Electrochemical DNA biosensor for detecting cancer biomarker related to glutathione S-transferase P1 (GSTP1) hypermethylation in real samples
Küçük Resim Yok
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Advanced Technology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An electrochemical genosensor for the detection of hypermethylation of the glutathione S-transferase P1 (GSTP1) gene, a specific marker of prostate cancer, was reported. This new sensor was used in combination with a single-use carbon graphite working electrode and differential pulse voltammetry, with the results of sample analysis based on the guanine oxidation signals obtained at +1.0 V before and after hybridization between probe and synthetic target or denatured PCR samples. The detected DNA hybridization was also characterized by electrochemical impedance spectroscopy with potassium ferri/ferrocyanide as a redox probe. The protocol consisted of 2 different modes: (i) capture probes selective for methylation-specific and unmethylated GSTP1 sequences were immobilized onto the sensor directly, and hybridization was formed on the electrode surface; (ii) probe/target or probe/noncomplementary target couples were mixed in solution phase, and the transducer was modified through simple adsorption. The limit of detection (S/N = 3) was calculated as 2.92 pmol of target sequence in a 100-mu l reaction volume. The optimum analytical detection parameters for the biosensor, as well as its future prospects, were also presented. (C) 2011 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Electrochemical DNA biosensor, Differential pulse voltammetry (DPV), Electrochemical impedance spectroscopy (EIS), Cancer marker, Glutathione S-transferase P1 hypermethylation
Kaynak
Biosensors & Bioelectronics
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
31
Sayı
1
