Osteoprotective effect of hormone therapy on bone microarchitecture before impaired bone mineral density in ovariectomized rats
Küçük Resim Yok
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Çalışmamızda ooferektomili sıçanlarda hormon replasman tedavisinin kemik mikromimari üzerine etkisini değerlendirmeyi amaçladık. Gereç ve Yöntemler: Çalışmanın hayvan etik komite onayı alındı. 17 ?-östradiol 50 ?g/kg ve medroksiprogesteron 2.5 mg/kg tedavisi ile; ooferektomili ve ooferektomi uygulanmayan sıçanlarda 20 günlük tedaviyi takiben kemik mikromimarisi ve kemik mineral yoğunluğa etkileri araştırıldı. Femoral ve lumbal kemik mineral yoğunluk ölçüm- leri yapıldı. Bulgular: Gruplar arasında kemik mineral yoğunluk ölçümleri değer- lendirildiğinde anlamlı fark bulunmadı. Ooferektomi uygulanmayan kontrol grubunda trabeküler yapılar diğer guplara göre anlamlı yüksek bulundu. Ayrıca ooferektomi uygulanmayan kontrol grubunda, diğer gruplara göre; osteoblast sayısı anlamlı yüksek ve osteoklast sayısı an- lamlı düşük bulundu. Sonuç: Tek başına östrojen yada progesteron ile kombine hormon te- davisinin; erken postmenapozal dönemde kemik mineral dansitesine yansımayan kemik kayıplarının önlenmesinde etkili olduğunu düşün- mekteyiz. (J Turkish-German Gynecol Assoc 2012; 13: 261-6)
Objective: We aimed to determine the effect of hormone replace- ment therapy on bone microarchitecture in ovariectomized rats. Material and Methods: In the Animal Ethics Committee approved- study, the effect of treatment with 17 β-estradiol 50 μg/kg and me- droxyprogesterone 2.5 mg/kg on bone architecture and bone mineral density in rats versus ovariectomized control rats over the course of 20 days were evaluated. Femoral and lumbar bone mineral density levels and morphometric measurements were performed. Results: There were no significant differences in the femoral and lumbar bone mineral density levels between the groups. In the intact control group, the trabecular structures were significantly superior to those in the other groups. Additionally, the osteoblast count was significantly higher while the osteoclast count was significantly lower than in all other groups. Two parameters reflecting trabecular bone microarchitecture, which include the trabecular count and the tra- becular area, demonstrated significant improvement in the hormone replacement group when compared to the ovariectomized control group. In the hormone replacement groups, the osteoblast count was significantly higher while the osteoclast count was significantly lower than in the ovariectomized control group. Conclusion: We suggest that offering estrogen alone or in combi- nation with progestogen can be a beneficial approach in preventing early postmenopausal bone loss regardless of bone mineral density. (J Turkish-German Gynecol Assoc 2012; 13: 261-6)
Objective: We aimed to determine the effect of hormone replace- ment therapy on bone microarchitecture in ovariectomized rats. Material and Methods: In the Animal Ethics Committee approved- study, the effect of treatment with 17 β-estradiol 50 μg/kg and me- droxyprogesterone 2.5 mg/kg on bone architecture and bone mineral density in rats versus ovariectomized control rats over the course of 20 days were evaluated. Femoral and lumbar bone mineral density levels and morphometric measurements were performed. Results: There were no significant differences in the femoral and lumbar bone mineral density levels between the groups. In the intact control group, the trabecular structures were significantly superior to those in the other groups. Additionally, the osteoblast count was significantly higher while the osteoclast count was significantly lower than in all other groups. Two parameters reflecting trabecular bone microarchitecture, which include the trabecular count and the tra- becular area, demonstrated significant improvement in the hormone replacement group when compared to the ovariectomized control group. In the hormone replacement groups, the osteoblast count was significantly higher while the osteoclast count was significantly lower than in the ovariectomized control group. Conclusion: We suggest that offering estrogen alone or in combi- nation with progestogen can be a beneficial approach in preventing early postmenopausal bone loss regardless of bone mineral density. (J Turkish-German Gynecol Assoc 2012; 13: 261-6)
Açıklama
Anahtar Kelimeler
Kadın Hastalıkları ve Doğum
Kaynak
Journal of the Turkish-German Gynecological Association
WoS Q Değeri
Scopus Q Değeri
Cilt
13
Sayı
4
