Graphene oxide has a neuroprotective effect against glutamate-induced excitoxicity on B35 neuroblastoma cell line*
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives:Graphene is a quasi-two-dimensional material with unique electrical and chemical properties. In terms of biomedical applications of graphene, nervous system would be an ideal breakthrough model because neural cells are electroactive. Extreme glutamate concentrations cause excitotoxicity. In this study, we aimed to investigate if graphene can increasethe resistance to glutamate stress in B35 rat neuroblastoma cells as a cultured cell model for central nervous system neurons. Methods: B35 neuroblastoma cells were grown in DMEM-F12 growth medium containing 10% fetal bovine serum.Graphene oxide (GO) powder was coated onto glass slides with chitosan as a thin film. B35 cells were cultured on GO films.Cells cultivated on glass slides were used as controls. After 24 h of cell culture, L-glutamine induced excitotoxicity wasimposed on B35 cells. After 24 h of glutamate-induced stress, cell morphology was examined by scanning electronmicroscopy. Cell viability was measured with MTT assay.Results: The effects of glutamate stress on cell viability were visible as early as 1 h. The cell viability on GO films was higher than that on glass slides, and cells recovered from stress within 6 h on GO surfaces. After 24 h, viability on glass surfaceswas 54% lower than that on GO surfaces; these findings were supported with cell morphology observations.. Conclusion:The results of this study showed that GO has a protective role in reducing glutamate-induced excitotoxicity inB35 cell culture, indicating a potential use of GO for treatment of excitotoxicity induced neurodegenerative diseases.
Objectives:Graphene is a quasi-two-dimensional material with unique electrical and chemical properties. In terms of biomedical applications of graphene, nervous system would be an ideal breakthrough model because neural cells are electroactive. Extreme glutamate concentrations cause excitotoxicity. In this study, we aimed to investigate if graphene can increasethe resistance to glutamate stress in B35 rat neuroblastoma cells as a cultured cell model for central nervous system neurons. Methods: B35 neuroblastoma cells were grown in DMEM-F12 growth medium containing 10% fetal bovine serum.Graphene oxide (GO) powder was coated onto glass slides with chitosan as a thin film. B35 cells were cultured on GO films.Cells cultivated on glass slides were used as controls. After 24 h of cell culture, L-glutamine induced excitotoxicity wasimposed on B35 cells. After 24 h of glutamate-induced stress, cell morphology was examined by scanning electronmicroscopy. Cell viability was measured with MTT assay.Results: The effects of glutamate stress on cell viability were visible as early as 1 h. The cell viability on GO films was higher than that on glass slides, and cells recovered from stress within 6 h on GO surfaces. After 24 h, viability on glass surfaceswas 54% lower than that on GO surfaces; these findings were supported with cell morphology observations.. Conclusion:The results of this study showed that GO has a protective role in reducing glutamate-induced excitotoxicity inB35 cell culture, indicating a potential use of GO for treatment of excitotoxicity induced neurodegenerative diseases.
Objectives:Graphene is a quasi-two-dimensional material with unique electrical and chemical properties. In terms of biomedical applications of graphene, nervous system would be an ideal breakthrough model because neural cells are electroactive. Extreme glutamate concentrations cause excitotoxicity. In this study, we aimed to investigate if graphene can increasethe resistance to glutamate stress in B35 rat neuroblastoma cells as a cultured cell model for central nervous system neurons. Methods: B35 neuroblastoma cells were grown in DMEM-F12 growth medium containing 10% fetal bovine serum.Graphene oxide (GO) powder was coated onto glass slides with chitosan as a thin film. B35 cells were cultured on GO films.Cells cultivated on glass slides were used as controls. After 24 h of cell culture, L-glutamine induced excitotoxicity wasimposed on B35 cells. After 24 h of glutamate-induced stress, cell morphology was examined by scanning electronmicroscopy. Cell viability was measured with MTT assay.Results: The effects of glutamate stress on cell viability were visible as early as 1 h. The cell viability on GO films was higher than that on glass slides, and cells recovered from stress within 6 h on GO surfaces. After 24 h, viability on glass surfaceswas 54% lower than that on GO surfaces; these findings were supported with cell morphology observations.. Conclusion:The results of this study showed that GO has a protective role in reducing glutamate-induced excitotoxicity inB35 cell culture, indicating a potential use of GO for treatment of excitotoxicity induced neurodegenerative diseases.
Açıklama
Anahtar Kelimeler
Anatomi ve Morfoloji
Kaynak
Anatomy
WoS Q Değeri
Scopus Q Değeri
Cilt
9
Sayı
3
