Combination Therapy with KRAS and P38 alpha siRNA Suppresses Colorectal Cancer Growth and Development in SW480 Cell Line
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose Colorectal cancer (CRC) is one of the most prevalence malignancies in a different society with a high rate of death. The KRAS and p38 alpha axes have critical roles in the development, migration, and growth of numerous tumors, such as colorectal malignancy. KRAS mutation acts as an oncogene in various cancers and is correlated with the poor prognosis in colorectal tumors. Also, p38 alpha plays different roles and exhibits tissue-dependent activity. In some tissues act as an oncogene while in others act as a tumor suppressor. In this research, we try to understand the effect of the P38 alpha and KRAS genes suppression by specific siRNAs on the SW480 cell line progression. Methods We evaluate the impact of the P38 alpha and KRAS gene knockdown by special siRNA on the growth and development of the SW480 cell line. SW480 cell line was treated with KRAS and P38 alpha siRNAs, and the cell viability, gene expression, migration ability, and rate of apoptosis were evaluated with MTT assay, real-time PCR, scratch test, and flow cytometry. Results After treatment of the cancer cell with KRAs and P38 alpha siRNAs, cell viability reduced to 29.16%. Also, the expression levels of the KRAS and P38 alpha genes reduced to 26.34% and 16.06%, respectively. Apoptosis rate after combination therapy with KRAS and P38 alpha siRNAs increased to 72.1. Also, we found that these siRNAs suppress cell migration in SW480 cell lines. Conclusion The current study showed that combination therapy with p38 alpha and KRAS siRNA may be considered a novel therapy for colorectal tumor in future.
Açıklama
Anahtar Kelimeler
Colorectal cancer, KRAS, P38, siRNA, Mutation Status, P38, Inhibition, Knockdown, Prognosis
Kaynak
Journal of Gastrointestinal Cancer
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
53
Sayı
3
