A novel non-competitive amperometric immunosensor by using thiourea- glutaraldehyde-modified gold electrode for immunoglobulin M detection
Küçük Resim Yok
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Informa Healthcare
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel non-competitive amperometric immunosensor based on a self-assembled monolayer (SAM) of thiourea modified by a polymeric Schiff's base of glutaraldehyde on gold electrode has been developed for determination of IgM. Alkaline phosphatase (ALP)-conjugated monoclonal anti-mouse immunoglobulin M (IgM) antibody was selectively bound to IgM molecules onto the surface of the electrode. Electrochemical response arising from the catalytic reaction of alkaline phosphatase enzyme. Its reaction with various phosphates such as p-aminophenyl phosphate and p-nitrophenyl phosphatase (p-NPP) generates the electrochemically active products p-aminophenol (p-AP) and p-nitrophenol (p-NP), respectively.
Açıklama
Anahtar Kelimeler
alkaline phosphatase, IgM, immunosensor, self assembled monolayer, thiourea
Kaynak
Artificial Cells Nanomedicine and Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
41
Sayı
6
