Enzyme-linked Electrochemical Detection of DNA Hybridization at the Surface of Pencil Graphite Electrode
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Lenka Srsenova-Best Servis
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Enzyme-linked electrochemical detection systems proved to be potent tools for various bioassay, including DNA hybridization assays and immunoassays. Previously we presented a simple technique using target DNA adsorbed on pyrolytic graphite electrode surface and biotinylated oligonucleotide probe to which alkaline phosphatase was attached via streptavidine linker. Here we used analogous system in combination with disposable pencil graphite electrodes. We demonstrate a facile and reproducible detection of DNA hybridization in a wide range of target concentrations using natural PCR-amplified DNA targets.
Açıklama
33rd International Conference on Modern Electrochemical Methods -- MAY 20-24, 2013 -- Jetrichovice, CZECH REPUBLIC
Anahtar Kelimeler
DNA hybridization, Electrochemical DNA sensors, Biotin label, Streptavidin alkaline phosphatase, p53
Kaynak
Xxxiii Moderni Elektrochemicke Metody
WoS Q Değeri
N/A
