Supercritical fluid extraction of phycocyanin and investigation of cytotoxicity on human lung cancer cells
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, a step-by-step optimization approach was carried out for supercritical fluid extraction (SCF) of phycocyanin (PC) from Spirulina platefuls, where pressure, temperature, co-solvent ratio and process duration were investigated. The elicited parameters were 250 bar, 60 degrees C, 10% ethanol as co-solvent at a dynamic duration of 45 min yielding PC amount of 90.74% and PC purity of 75.12%. Extracts obtained with SCF were tested against lung cancer cell line (A549) to determine cytotoxicity and compared to that obtained from solvent extraction. SCF extract had an IC50 value of 26.82 mu g/mL, whereas that value was 36.94 mu g/mL for solvent extract indicating a lower cytotoxic effect. Overall, the results revealed significant information for PC extraction using supercritical fluids as an alternative to conventional methods and the possibility to include PC in daily diet for preventive purposes against highly lethal lung cancer. (C) 2015 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Supercritical fluid extraction, Spirulina platensis, Phycocyanin, Cytotoxicity, A549 lung cancer cells
Kaynak
Journal of Supercritical Fluids
WoS Q Değeri
Q1
Scopus Q Değeri
N/A
Cilt
108
